State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Third Institute of Oceanography, State Oceanic Administration, 184 Daxue Road, Xiamen, 361005, People's Republic of China.
Sci Rep. 2018 Jan 11;8(1):530. doi: 10.1038/s41598-017-18841-6.
Nine new guaianes (graphostromanes A-I, 1-9) were isolated from the deep-sea-derived fungus Graphostroma sp. MCCC 3A00421, along with four known ones (10-13). The relative configurations were established mainly by detailed analysis of the NMR and HRESIMS data, while the absolute configurations were assigned using the X-ray crystallography and modified Mosher's method. All isolates were evaluated for their inhibitory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages. Graphostromanes F (6) showed remarkable inhibitory effect with an IC value of 14.2 μM, which was even stronger than that of aminoguanidine, a positive control with an IC value of 23.4 μM.
从深海来源真菌 Graphostroma sp. MCCC 3A00421 中分离得到了 9 个新的瓜环（graphostromanes A-I，1-9），以及 4 个已知化合物（10-13）。通过详细分析 NMR 和 HRESIMS 数据确定了相对构型，而绝对构型则是通过 X 射线晶体学和改进的 Mosher 方法确定的。所有分离物均进行了评估，以检测其对 RAW264.7 巨噬细胞中脂多糖（LPS）诱导的一氧化氮（NO）产生的抑制作用。Graphostromanes F（6）表现出显著的抑制作用，IC 值为 14.2 μM，甚至强于阳性对照物氨基胍，其 IC 值为 23.4 μM。
