Wei Han, Xu Ya-ming, Espinosa-Artiles Patricia, Liu Manping X, Luo Jiang-Guang, U'Ren Jana M, Arnold A Elizabeth, Gunatilaka A A Leslie
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Southwest Center for Natural Products Research and Commercialization, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States; State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China.
Phytochemistry. 2015 Oct;118:102-8. doi: 10.1016/j.phytochem.2015.08.010. Epub 2015 Aug 28.
Oxygenated guaiane-type sesquiterpenes, xylaguaianols A-D (1-4), an iso-cadinane-type sesquiterpene isocadinanol A (5), and an α-pyrone 9-hydroxyxylarone (6), together with five known sesquiterpenes (7-11), and four known cytochalasins (12-15) were isolated from a culture broth of Xylaria sp. NC1214, a fungal endophyte of the moss Hypnum sp. The structures of all compounds were elucidated by the analysis of their spectroscopic data and relative configurations of 1-5 were determined with the help of NMR NOESY experiments. Cytochalasins C (12), D (13), and Q (14) were investigated for their cytotoxic activity against five tumor cell lines. Cytochalasin D showed significant cytotoxicity against all five cell lines, with IC50s ranging from 0.22 to 1.44 μM, whereas cytochalasins C and Q exhibited moderate, but selective cytotoxicity.
从苔藓植物Hypnum sp.的真菌内生菌木层孔菌Xylaria sp. NC1214的培养液中分离得到了氧化愈创木烷型倍半萜类化合物木层孔愈创木醇A-D（1-4）、一种异杜松烷型倍半萜异杜松醇A（5）、一种α-吡喃酮9-羟基拉里酮（6），以及五种已知的倍半萜（7-11）和四种已知的细胞松弛素（12-15）。所有化合物的结构均通过光谱数据分析得以阐明，1-5的相对构型借助核磁共振NOESY实验得以确定。研究了细胞松弛素C（12）、D（13）和Q（14）对五种肿瘤细胞系的细胞毒性活性。细胞松弛素D对所有五种细胞系均表现出显著的细胞毒性，IC50值范围为0.22至1.44 μM，而细胞松弛素C和Q表现出中等但具有选择性的细胞毒性。
