Gu Zhouhang, Liu Tingli, Ding Bo, Li Fangfang, Wang Qian, Qian Shasha, Ye Fei, Chen Tianzi, Yang Yuwen, Wang Jinyan, Wang Guoliang, Zhang Baolong, Zhou Xueping
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Science, Zhejiang Sci-Tech University, Hangzhou, China.
Front Plant Sci. 2017 Dec 13;8:2133. doi: 10.3389/fpls.2017.02133. eCollection 2017.
Lysin-motif (LysM) receptor kinases (LYKs) play essential roles in recognition of chitin and activation of defense responses against pathogenic fungi in the model plants and rice. The function of LYKs in non-model plants, however, remains elusive. In the present work, we found that the transcription of two LYK-encoding genes from cotton, and , was induced after infection. Virus-induced gene silencing (VIGS) of and in cotton plants compromises resistance to . As putative pattern recognition receptors (PRRs), both Gh-LYK1 and Gh-LYK2 are membrane-localized, and all three LysM domains of Gh-LYK1 and Gh-LYK2 are required for their chitin-binding ability. However, since Gh-LYK2, but not Gh-LYK1, is a pseudo-kinase and, on the other hand, the ectodomain (ED) of Gh-LYK2 can induce reactive oxygen species (ROS) burst in planta, Gh-LYK2 and Gh-LYK1 may contribute differently to cotton defense. Taken together, our results establish that both Gh-LYK1 and Gh-LYK12 are required for defense against in cotton, possibly through different mechanisms.
赖氨酰基序（LysM）受体激酶（LYKs）在模式植物和水稻中识别几丁质以及激活针对致病真菌的防御反应中发挥着重要作用。然而，LYKs在非模式植物中的功能仍不清楚。在本研究中，我们发现棉花中两个编码LYK的基因Gh-LYK1和Gh-LYK2的转录在黄萎病菌感染后被诱导。在棉花植株中对Gh-LYK1和Gh-LYK2进行病毒诱导的基因沉默（VIGS）会损害对黄萎病菌的抗性。作为假定的模式识别受体（PRRs），Gh-LYK1和Gh-LYK2都定位于细胞膜，并且Gh-LYK1和Gh-LYK2的所有三个LysM结构域都是它们几丁质结合能力所必需的。然而，由于Gh-LYK2是一个假激酶而Gh-LYK1不是，并且另一方面，Gh-LYK2的胞外结构域（ED）可以在植物中诱导活性氧（ROS）爆发，因此Gh-LYK2和Gh-LYK1可能对棉花防御有不同的贡献。综上所述，我们的结果表明，Gh-LYK1和Gh-LYK12对于棉花抵御黄萎病菌都是必需的，可能是通过不同的机制。
