Department of Environmental Science, Graduate School of Agriculture, Saga University, Honjo 1, Saga, 840-8502, Japan.
Department of Social Medicine, Saga University School of Medicine, Saga, Japan.
Environ Sci Pollut Res Int. 2018 Jun;25(17):16309-16315. doi: 10.1007/s11356-017-1031-x. Epub 2018 Jan 13.
Risk assessment of infant using a realistic persistent organic pollutant (POP) exposure through breast milk is essential to devise future regulation of POPs. However, recent investigations have demonstrated that POP levels in breast milk collected from the same mother showed a wide range of variation daily and monthly. To estimate the appropriate sample size of breast milk from the same mother to obtain reliable POP concentrations, breast milk samples were collected from five mothers living in Japan from 2006 to 2012. Milk samples from each mother were collected 3 to 6 times a day through 3 to 7 days consecutively. Food samples as the duplicated method were collected from two mothers during the period of breast milk sample collection. Those were employed for POP (PCBs, DDTs, chlordanes, and HCB) analysis. PCB concentrations detected in breast milk samples showed a wide range of variation which was maximum 63 and 60% of relative standard deviation (RSD) in lipid and wet weight basis, respectively. The time course trend of those variations among the mothers did not show any typical pattern. A larger amount of PCB intake through food seemed to affect 10 h after those concentrations in breast milk in lipid weight basis. Intraclass correlation coefficient (ICC) analyses indicated that the appropriate sample size for good reproducibility of POP concentrations in breast milk required at least two samples for lipid and wet weight basis.
通过母乳对婴儿进行持久性有机污染物（POP）暴露风险评估对于制定未来的 POP 法规至关重要。然而，最近的调查表明，从同一母亲收集的母乳中的 POP 水平在每日和每月都存在广泛的变化。为了估计从同一母亲获得可靠 POP 浓度的母乳的适当样本量，从 2006 年至 2012 年在日本收集了 5 位母亲的母乳样本。通过连续 3 至 7 天每天收集 3 至 6 次每个母亲的奶样。在收集母乳样本期间，从两位母亲收集了食物样本作为重复方法。这些样本用于 POP（多氯联苯、滴滴涕、氯丹和 HCB）分析。母乳样本中检测到的 PCB 浓度变化范围很广，在脂质和湿重基础上的相对标准偏差（RSD）分别最大为 63%和 60%。这些变化在母亲之间的时间过程趋势没有显示出任何典型模式。通过食物摄入更多的 PCB 似乎会影响脂质重量基础上母乳中这些浓度 10 小时后的浓度。组内相关系数（ICC）分析表明，对于母乳中 POP 浓度具有良好重现性的适当样本量，脂质和湿重基础至少需要两个样本。
