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洛塔克湖野生稻草(Zizania latifolia)经稀酸预处理对酶解的影响。

Effect of dilute acid pretreatment of wild rice grass (Zizania latifolia) from Loktak Lake for enzymatic hydrolysis.

机构信息

Institute of Bioresources and Sustainable Development (IBSD), A National Institute under Department of Biotechnology Govt. of India, Takyelpat, Imphal 795001, Manipur, India.

Institute of Bioresources and Sustainable Development (IBSD), A National Institute under Department of Biotechnology Govt. of India, Takyelpat, Imphal 795001, Manipur, India.

出版信息

Bioresour Technol. 2018 Apr;253:252-255. doi: 10.1016/j.biortech.2018.01.048. Epub 2018 Jan 11.

DOI:10.1016/j.biortech.2018.01.048
PMID:29353753
Abstract

Zizania latifolia commonly known as wild rice grass which is available in huge quantities in Loktak Lake is a major concern as it occupies a large area of the Lake and causing a several environmental problems. The investigation of present study was to evaluate possibilities of using Zizania latifolia as feed stock for bioethanol production. The method involved the pretreatment with dilute acid or alkali followed by enzymatic hydrolysis with commercial cellulase. Acid pretreatment was performed with 10% biomass loading with different concentration of acids (0.4-2% w/v) and alkali (0.25-1.5% w/v). Maximum sugar release of 457 mg/g was obtained from 10% biomass loading and 2% w/v of acids. Alkali pretreatment is not effective for this grass. Physicochemical characterization of untreated and treated biomass was carried out by XRD, FTIR, SEM and corresponding alterations in the chemical composition were also monitored. Results showed the feasibility of this grass as biofuel (bioethanol) feed stock and can be potential approach to address the sustainable utilization phumdis grasses of Loktak Lake for the production of value added product.

摘要

菰俗称野茭白,在洛塔湖中有大量分布,是主要关注的问题,因为它占据了湖泊的很大面积,并导致了一些环境问题。本研究的调查旨在评估将菰作为生物乙醇生产原料的可能性。该方法涉及用稀酸或碱预处理,然后用商业纤维素酶进行酶水解。酸预处理是在 10%生物质负载下进行的,使用不同浓度的酸(0.4-2%w/v)和碱(0.25-1.5%w/v)。从 10%生物质负载和 2%w/v 的酸中获得了最大的 457mg/g 糖释放量。该草的碱预处理无效。通过 XRD、FTIR、SEM 对未处理和处理后的生物质进行了物理化学特性分析,并监测了化学成分的相应变化。结果表明,这种草作为生物燃料(生物乙醇)原料是可行的,并且可能是解决洛塔湖湿地草可持续利用问题的一种潜在方法,以生产高附加值产品。

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