Hassan Karl A, Liu Qi, Elbourne Liam D H, Ahmad Irshad, Sharples David, Naidu Varsha, Chan Chak Lam, Li Liping, Harborne Steven P D, Pokhrel Alaska, Postis Vincent L G, Goldman Adrian, Henderson Peter J F, Paulsen Ian T
School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia; School of BioMedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK; Department of Chemistry and Biomolecular Science, Macquarie University, North Ryde, NSW, Australia.
Department of Chemistry and Biomolecular Science, Macquarie University, North Ryde, NSW, Australia.
Res Microbiol. 2018 Sep-Oct;169(7-8):450-454. doi: 10.1016/j.resmic.2018.01.001. Epub 2018 Feb 2.
The proteobacterial antimicrobial compound efflux (PACE) family of transport proteins was only recently described. PACE family transport proteins can confer resistance to a range of biocides used as disinfectants and antiseptics, and are encoded by many important Gram-negative human pathogens. However, we are only just beginning to appreciate the range of functions and the mechanism(s) of transport operating in these proteins. Genes encoding PACE family proteins are typically conserved in the core genomes of bacterial species rather than on recently acquired mobile genetic elements, suggesting that they confer important core functions in addition to biocide resistance. Three-dimensional structural information is not yet available for PACE family proteins. However, PACE proteins have several very highly conserved amino acid sequence motifs that are likely to be important for substrate transport. PACE proteins also display strong amino acid sequence conservation between their N and C-terminal halves, suggesting that they evolved by duplication of an ancestral protein comprised of two transmembrane helices. In light of their drug resistance functions in Gram-negative pathogens, PACE proteins should be the subject of detailed future investigation.
细菌抗菌化合物外排（PACE）家族转运蛋白直到最近才被描述。PACE家族转运蛋白可赋予对一系列用作消毒剂和防腐剂的杀菌剂的抗性，并且由许多重要的革兰氏阴性人类病原体编码。然而，我们才刚刚开始认识到这些蛋白所具有的功能范围以及转运机制。编码PACE家族蛋白的基因通常在细菌物种的核心基因组中保守，而不是在最近获得的可移动遗传元件上，这表明它们除了具有抗杀菌剂功能外，还赋予重要的核心功能。目前尚无PACE家族蛋白的三维结构信息。然而，PACE蛋白有几个高度保守的氨基酸序列基序，可能对底物转运很重要。PACE蛋白在其N端和C端之间也表现出很强的氨基酸序列保守性，这表明它们是由一个由两个跨膜螺旋组成的祖先蛋白复制进化而来的。鉴于它们在革兰氏阴性病原体中的耐药功能，PACE蛋白应该是未来详细研究的对象。
