Vaishampayan Ankita, de Jong Anne, Wight Darren J, Kok Jan, Grohmann Elisabeth
Life Sciences and Technology, Beuth University of Applied Sciences Berlin, Berlin, Germany.
Department of Molecular Genetics, University of Groningen, Groningen, Netherlands.
Front Microbiol. 2018 Feb 15;9:221. doi: 10.3389/fmicb.2018.00221. eCollection 2018.
Methicillin-resistant (MRSA) has become an important cause of hospital-acquired infections worldwide. It is one of the most threatening pathogens due to its multi-drug resistance and strong biofilm-forming capacity. Thus, there is an urgent need for novel alternative strategies to combat bacterial infections. Recently, we demonstrated that a novel antimicrobial surface coating, AGXX, consisting of micro-galvanic elements of the two noble metals, silver and ruthenium, surface-conditioned with ascorbic acid, efficiently inhibits MRSA growth. In this study, we demonstrated that the antimicrobial coating caused a significant reduction in biofilm formation (46%) of the clinical MRSA isolate, 04-02981. To understand the molecular mechanism of the antimicrobial coating, we exposed 04-02981 for different time-periods to the coating and investigated its molecular response via next-generation RNA-sequencing. A conventional antimicrobial silver coating served as a control. RNA-sequencing demonstrated down-regulation of many biofilm-associated genes and of genes related to virulence of . The antimicrobial substance also down-regulated the two-component quorum-sensing system suggesting that it might interfere with quorum-sensing while diminishing biofilm formation in 04-02981.
耐甲氧西林金黄色葡萄球菌（MRSA）已成为全球医院获得性感染的重要原因。由于其多重耐药性和强大的生物膜形成能力，它是最具威胁性的病原体之一。因此，迫切需要新的替代策略来对抗细菌感染。最近，我们证明了一种新型抗菌表面涂层AGXX，它由银和钌这两种贵金属的微电流元素组成，并经抗坏血酸表面处理，能有效抑制MRSA的生长。在本研究中，我们证明了这种抗菌涂层可使临床MRSA分离株04 - 02981的生物膜形成显著减少（46%）。为了解这种抗菌涂层的分子机制，我们将04 - 02981在不同时间段暴露于该涂层，并通过下一代RNA测序研究其分子反应。一种传统的抗菌银涂层用作对照。RNA测序表明许多与生物膜相关的基因以及与04 - 嗯，这里原文似乎有误，可能是与04 - 02981毒力相关的基因被下调。这种抗菌物质还下调了双组分群体感应系统，这表明它可能在减少04 - 02981生物膜形成的同时干扰群体感应。
