Heck Christian, Kanehira Yuya, Kneipp Janina, Bald Ilko
Department of Chemistry-Physical Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter Str. 11, 12489, Berlin, Germany.
Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7444-7447. doi: 10.1002/anie.201801748. Epub 2018 May 2.
This study demonstrates the bottom-up synthesis of silver nanolenses. A robust coating protocol enabled the functionalization of differently sized silver nanoparticles with DNA single strands of orthogonal sequence. Coated particles 10 nm, 20 nm, and 60 nm in diameter were self-assembled by DNA origami scaffolds to form silver nanolenses. Single molecules of the protein streptavidin were selectively placed in the gap of highest electric field enhancement. Streptavidin labelled with alkyne groups served as model analyte in surface-enhanced Raman scattering (SERS) experiments. By correlated Raman mapping and atomic force microscopy, SERS signals of the alkyne labels of a single streptavidin molecule, from a single silver nanolens, were detected. The discrete, self-similar aggregates of solid silver nanoparticles are promising for plasmonic applications.
本研究展示了银纳米透镜的自下而上合成。一种稳健的涂层方案能够用正交序列的DNA单链对不同尺寸的银纳米颗粒进行功能化。直径为10 nm、20 nm和60 nm的包覆颗粒通过DNA折纸支架自组装形成银纳米透镜。蛋白质链霉亲和素的单分子被选择性地放置在电场增强最高的间隙中。用炔基标记的链霉亲和素用作表面增强拉曼散射（SERS）实验中的模型分析物。通过相关的拉曼映射和原子力显微镜，检测到了来自单个银纳米透镜的单个链霉亲和素分子炔基标记的SERS信号。固态银纳米颗粒的离散、自相似聚集体在等离子体应用方面很有前景。
