Jiangsu Key Laboratory for High Technology Research of TCM Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
College of Pharmacy and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, 712046, Shaanxi Province, China.
J Pharm Biomed Anal. 2018 Jun 5;155:169-176. doi: 10.1016/j.jpba.2018.03.035. Epub 2018 Apr 3.
The dried roots of Euphorbia kansui T.N. Liou ex T.P. Wang have been traditionally used for edema in China. However, the severe toxicity caused by Euphorbia kansui has seriously restricted its clinical application. Therefore, in order to study the material basis of the toxicity attenuation effect of processing with vinegar, a rapid, sensitive, validated and reliable UPLC-MS/MS method was developed to determine twelve compounds in ethyl acetate extracts of Euphorbia kansui before and after fry-baked with vinegar simultaneously. Meanwhile, the study of their toxic effect on zebrafish was conducted. Chromatographic separation was accomplished on Waters BEH C UPLC column. 0.3% formic acid in water and acetonitrile were used as mobile phase with a flow rate of 0.40 mL/min and a temperature at 30 °C. The analysis was performed in multiple reaction monitoring (MRM) mode using positive electrospray ionization (ESI). Furthermore, the toxic research results indicated that the toxicity of Euphorbia kansui was decreased after vinegar-processed, which might because of the increase in the content of 5-O-benzoyl-20-deoxyingenol and the decrease in the contents of the remaining terpenoids in ethyl acetate extracts of Euphorbia kansui fry-baked with vinegar. This study demonstrated that the method used is a powerful approach to determine the content of twelve compounds that responsible for the toxic effect of Euphorbia kansui at the same instant. And provided the experimental evidence for the rationale behind the reduction of toxicity.
甘遂的干根在中国传统上用于水肿。然而，甘遂的严重毒性严重限制了其临床应用。因此，为了研究醋炙减毒效应的物质基础，开发了一种快速、灵敏、验证和可靠的 UPLC-MS/MS 方法，同时测定醋炙前后甘遂乙酸乙酯提取物中的 12 种化合物。同时，研究了它们对斑马鱼的毒性作用。色谱分离在 Waters BEH C UPLC 柱上进行。以 0.3%甲酸水和乙腈为流动相，流速为 0.40 mL/min，温度为 30°C。采用正电喷雾电离（ESI）进行多反应监测（MRM）模式分析。此外，毒性研究结果表明，甘遂经醋炙后毒性降低，这可能是由于醋炙甘遂乙酸乙酯提取物中 5-O-苯甲酰-20-去氧异育醇含量增加，其余萜类化合物含量降低所致。本研究表明，该方法是一种同时测定甘遂毒性相关 12 种化合物含量的有效方法，为甘遂减毒的合理性提供了实验依据。
