Department of Gynecology and Obstetrics, In Vitro Fertilization Unit, Süleyman Demirel University School of Medicine, Isparta, Turkey
Clinic of Gynecology and Obstetrics, University of Health Sciences, Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Turkey
Balkan Med J. 2018 Jul 24;35(4):306-310. doi: 10.4274/balkanmedj.2017.1140. Epub 2018 Apr 4.
Oxidative stress has been proposed as a potential trigger in the etiopathogenesis of polycystic ovary syndrome-related infertility. Thiol/disulphide homeostasis, a recently identified oxidative stress marker, is one of the antioxidant mechanism in humans with critical roles in folliculogenesis and ovulation.
To investigate follicular fluid thiol/disulphide homeostasis in the etiopathogenesis of polycystic ovary syndrome and to determine its association with in vitro fertilization outcome. The study procedures were approved by the local ethics committee.
Cross-sectional study.
Follicular fluid from 22 women with polycystic ovary syndrome and 20 ovulatory controls undergoing in vitro fertilization treatment was sampled. Thiol/disulphide homeostasis was analyzed via a novel spectrophotometric method.
Follicular native thiol levels, as well as the native thiol/total thiol ratio, were lower in the polycystic ovary syndrome group than in the non-polycystic ovary syndrome group (p=0.041 and p<0.0001, respectively). Disulphide levels, disulphide/native thiol, and disulphide/total thiol ratios were increased in the polycystic ovary syndrome group (p<0.0001). A positive correlation between the fertilization rate and native thiol (p=0.01, r=0.53) and total thiol (p=0.01, r=0.052) among polycystic ovary syndrome patients was found. A positive predictive effect of native thiol level on the fertilization rate in the polycystic ovary syndrome group was also found (p=0.03, β=0.45, 95% CI= 0.031-0.643).
Deterioration of thiol/disulphide homeostasis, especially elevated disulphide levels, could be one of the etiopathogenetic mechanisms in polycystic ovary syndrome. Increased native thiol levels are related to the fertilization rate among polycystic ovary syndrome patients and are positive predictors of the fertilization rate among polycystic ovary syndrome patients. Improvement of thiol/disulphide homeostasis could be important in the treatment of polycystic ovary syndrome to increase in vitro fertilization success.
氧化应激被认为是多囊卵巢综合征相关不孕发病机制中的一个潜在触发因素。硫醇/二硫键平衡是最近确定的氧化应激标志物之一，是人类中一种关键的抗氧化机制，在卵泡发生和排卵中起着重要作用。
研究多囊卵巢综合征发病机制中的卵泡液硫醇/二硫键平衡，并确定其与体外受精结局的关系。该研究程序得到了当地伦理委员会的批准。
横断面研究。
采集 22 例多囊卵巢综合征患者和 20 例接受体外受精治疗的排卵对照组的卵泡液。通过一种新的分光光度法分析硫醇/二硫键平衡。
多囊卵巢综合征组卵泡内源性硫醇水平以及内源性硫醇/总硫醇比值均低于非多囊卵巢综合征组（p=0.041 和 p<0.0001）。多囊卵巢综合征组中二硫键水平、二硫键/内源性硫醇和二硫键/总硫醇比值升高（p<0.0001）。多囊卵巢综合征患者的受精率与内源性硫醇（p=0.01，r=0.53）和总硫醇（p=0.01，r=0.052）呈正相关。还发现多囊卵巢综合征组内源性硫醇水平对受精率有正向预测作用（p=0.03，β=0.45，95%CI=0.031-0.643）。
硫醇/二硫键平衡恶化，尤其是二硫键水平升高，可能是多囊卵巢综合征发病机制之一。多囊卵巢综合征患者内源性硫醇水平升高与受精率相关，是其受精率的阳性预测指标。改善硫醇/二硫键平衡可能对提高体外受精成功率具有重要意义。
