Li Kang, Yuan Xiao-Xue, Sun He-Min, Zhao Long-Sheng, Tang Ruocong, Chen Zhi-Hua, Qin Qi-Long, Chen Xiu-Lan, Zhang Yu-Zhong, Su Hai-Nan
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Front Microbiol. 2018 Mar 29;9:620. doi: 10.3389/fmicb.2018.00620. eCollection 2018.
Peptidoglycan is the fundamental structural constituent of the bacterial cell wall. Despite many years of research, the architecture of peptidoglycan is still largely elusive. Here, we report the high-resolution architecture of peptidoglycan from the model Gram-positive bacterium . We provide high-resolution evidence of peptidoglycan architecture remodeling at different growth stages. Side wall peptidoglycan from strain AS1.398 changed from an irregular architecture in exponential growth phase to an ordered cable-like architecture in stationary phase. Thickness of side wall peptidoglycan was found to be related with growth stages, with a slight increase after transition to stationary phase. Septal disks were synthesized progressively toward the center, while the surface features were less clear than those imaged with side walls. Compared with previous studies, our results revealed slight differences in architecture of peptidoglycan from different strains, expanding our knowledge about the architectural features of peptidoglycan.
肽聚糖是细菌细胞壁的基本结构成分。尽管经过多年研究，肽聚糖的结构在很大程度上仍然难以捉摸。在此，我们报告了来自模式革兰氏阳性菌的肽聚糖的高分辨率结构。我们提供了不同生长阶段肽聚糖结构重塑的高分辨率证据。菌株AS1.398的侧壁肽聚糖从指数生长期的不规则结构转变为稳定期的有序缆状结构。发现侧壁肽聚糖的厚度与生长阶段有关，在转变为稳定期后略有增加。隔膜盘向中心逐渐合成，而其表面特征不如侧壁成像清晰。与先前的研究相比，我们的结果揭示了不同菌株的肽聚糖结构存在细微差异，扩展了我们对肽聚糖结构特征的认识。
