Pasdaran Ardalan, Delazar Abbas, Ayatollahi Seyed Abdulmajid, Pasdaran Arsalan
Medical Plant Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pharmacognosy, School of Pharmacy, Research and Development Center of Plants and Medicinal Chemistry, Guilan University of Medical Sciences, Rasht, Iran.
Iran J Pharm Res. 2017 Winter;16(1):338-346.
Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol), one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol) compound from the methanolic extract of aerial parts of using by high performance liquid chromatographyare based on isocratic and liner gradients by C18 column. The structure elucidations of the isolated compounds were performed by spectroscopic methods including1H-NMR, 13C-NMR, 2 D NMR technique such as HMBC( in deuterated methanol as solvent), GC-MS and UV, also methanolic extract and fractions( fractionated on solid phase extraction on C18 cartridge(Spack-C18)) of this plant was tested for free radical scavenging properties toward the 1, 1-diphenyl-1-picrylhydrazyl (DPPH), general toxicity (Brine shrimp toxicity assay) , insecticidal ( Contact toxicity insecticidal assay) and antimalarial activities (hemebiocrystallization inhibition assay).
采用高效液相色谱法，基于等度和线性梯度，通过C18柱从[植物名称]地上部分的甲醇提取物中分离出5种已知的环烯醚萜苷（玄参苷A、哈巴苷B、5-羟基马钱苷、8-O-乙酰哈巴苷和6-O-甲基-1-葡萄糖基梓醇）、1种苯乙醇苷（毛蕊花糖苷）和1种苯乙醇胺（2-(4-氯苄基氨基)乙醇）化合物。通过光谱方法对分离出的化合物进行结构解析，这些方法包括1H-NMR、13C-NMR、二维NMR技术如HMBC（以氘代甲醇为溶剂）、GC-MS和UV，此外，还测试了该植物的甲醇提取物和馏分（在C18柱固相萃取（Spack-C18）上进行分馏）对1,1-二苯基-2-苦基肼（DPPH）的自由基清除性能、一般毒性（卤虫毒性试验）、杀虫活性（接触毒性杀虫试验）和抗疟活性（血红素生物结晶抑制试验）。 （注：原文中“using by high performance liquid chromatographyare based on isocratic and liner gradients by C18 column”表述有误，推测正确表述可能是“using high performance liquid chromatography based on isocratic and linear gradients by C18 column” ，翻译时已按推测正确内容翻译。）
