Rembe Julian-Dario, Fromm-Dornieden Carolin, Stuermer Ewa Klara
In the Department of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Witten, Germany, Julian-Dario Rembe, MD, is a Physician; Carolin Fromm-Dornieden, PhD, is a Postdoctoral Researcher; and Ewa Klara Stuermer, Univ Prof Dr Med, is a Physician and Professor. Acknowledgments: The authors are grateful to their colleagues V. Koester and A. Richard for their assistance with the manuscript. The authors have disclosed no financial relationships related to this article. Submitted May 19, 2017; accepted in revised form November 17, 2017.
Adv Skin Wound Care. 2018 May;31(5):225-233. doi: 10.1097/01.ASW.0000531351.85866.d9.
Vitamins are essential for human health. In terms of local application for wound healing, vitamins' positive effect remains unclear. However, because of the regular appearance of nutritional deficiency in chronic wound patients, a favorable impact of locally applied vitamins can be hypothesized.
Vitamins B3, B5, B6, B7, B9, B10, B12, and C individually as well as different combinations of B vitamins were investigated regarding their ability to promote proliferation and migration of human skin fibroblasts and keratinocytes. Proliferation assays with and without bacterial challenge, immunocytochemical staining, and scratch assay were used to determine the most effective combination(s).
Some vitamin combinations showed a positive impact on proliferation, especially for keratinocytes after 72 hours. In terms of wound closure, the combinations B9 and B12; B3, B5, B6, and B10; and B3, B5, and B7 improved closure rates by 25% to 30%. The improved closure rates are also reflected by immunocytochemically detected upregulation of the migration marker CXCR4 for several combinations.
Certain combinations of B vitamins demonstrate a positive influence on human keratinocytes and fibroblasts. Vitamins especially promoted fibroblast migration, and a statistically significant induction of keratinocyte proliferation was observed. Therefore, local vitamin application could benefit the physiologic wound healing process.
维生素对人体健康至关重要。就局部应用于伤口愈合而言，维生素的积极作用仍不明确。然而，由于慢性伤口患者经常出现营养缺乏，因此可以推测局部应用维生素会产生有利影响。
分别研究了维生素B3、B5、B6、B7、B9、B10、B12和C以及不同组合的B族维生素促进人皮肤成纤维细胞和角质形成细胞增殖和迁移的能力。采用有无细菌攻击的增殖试验、免疫细胞化学染色和划痕试验来确定最有效的组合。
一些维生素组合对增殖有积极影响，尤其是72小时后的角质形成细胞。在伤口闭合方面，B9和B12组合；B3、B5、B6和B10组合；以及B3、B5和B7组合使闭合率提高了25%至30%。几种组合的迁移标志物CXCR4免疫细胞化学检测上调也反映了闭合率的提高。
某些B族维生素组合对人角质形成细胞和成纤维细胞有积极影响。维生素尤其促进了成纤维细胞迁移，并观察到角质形成细胞增殖有统计学意义的诱导。因此，局部应用维生素可能有益于生理性伤口愈合过程。
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