School of Chemical Engineering and Technology, Shaanxi Key Laboratory of Energy Chemical Process Intensification, Institute of Polymer Science in Chemical Engineering , Xi'an Jiao Tong University , Xi'an Shaanxi 710049 , P. R. China.
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology , Fourth Military Medical University , Xi'an , Shaanxi 710032 , China.
ACS Appl Mater Interfaces. 2018 May 23;10(20):17018-17027. doi: 10.1021/acsami.8b01740. Epub 2018 May 12.
Microbial infection has been considered as one of the most critical challenges in bioengineering applications especially in tissue regeneration, which engenders severe threat to public health. Herein, a hydrogel performing properties of rapid self-healing, on-demand antibiosis and controlled cargo release was fabricated by a simple assembly of Fe complex as the cross-linker and hyaluronic acid as the gel network. This hydrogel is able to locally degrade and release Fe to kill bacteria as needed because of hyaluronidase excreted by surrounding bacteria, resulting in efficient antibacterial activity against different types of bacteria. The sustained release property of certain types of growth factors was also observed from this hydrogel owing to its dense network. Moreover, this hydrogel could repeatedly heal itself in minutes because of the coordination interaction between Fe and COOH, exhibiting good potential in bioengineering applications on the exposed tissue, where the materials are easily damaged during daily life. When topically applied onto damaged mouse skin with infection of Staphylococcus aureus, the hydrogel is able to inhibit microbial infections, meanwhile promoting cutaneous regeneration, which formed new skin with no inflammation within a 10 day treatment. These results demonstrate the potential application of this self-healing hydrogel for the integrated therapy of antibiosis and tissue regeneration.
微生物感染被认为是生物工程应用中最关键的挑战之一，特别是在组织再生方面，这对公共健康构成了严重威胁。在这里，通过简单地组装 Fe 配合物作为交联剂和透明质酸作为凝胶网络，制备了一种具有快速自修复、按需抗菌和控制药物释放性能的水凝胶。由于周围细菌分泌的透明质酸酶，这种水凝胶能够局部降解并按需释放 Fe 来杀死细菌，从而对不同类型的细菌表现出有效的抗菌活性。由于其密集的网络，还观察到这种水凝胶对某些类型的生长因子的持续释放特性。此外，由于 Fe 和 COOH 之间的配位相互作用，这种水凝胶可以在几分钟内反复自我修复，在暴露组织的生物工程应用中具有良好的应用潜力，因为在日常生活中这些材料很容易受损。当将该水凝胶局部应用于金黄色葡萄球菌感染的受损小鼠皮肤时，它能够抑制微生物感染，同时促进皮肤再生，在 10 天的治疗后形成没有炎症的新皮肤。这些结果表明，这种自修复水凝胶在抗菌和组织再生的综合治疗中有潜在的应用。
