Department of Biological Sciences, Faculty of Science, University of Jordan, Amman-11942, Jordan.
Molecules. 2010 Mar 12;15(3):1811-24. doi: 10.3390/molecules15031811.
Antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. Ethanol, methanol, aqueous, butanol, and n-hexane extracts were tested against three gram negative, two gram positive bacteria, and two fungi. Cytotoxicity and phytochemical screening were determined using MTT and TLC assays, respectively. Of the fifty one extracts, twenty two showed activities against different microorganisms with MICs ranging from 62.5 to 1000 microg/mL. The highest activity (100% inhibition) was for a butanol extract of Rosa damascena receptacles against Salmonella typhimurium and Bacillus cereus (MIC of 62.5 and 250 microg/mL) respectively. Butanol extract of Narcissus tazetta aerial parts and aqueous extract of Rosa damascena receptacles were both active against Candida albicans (MIC of 125 microg/mL). Methicillin-resistant Staphylococcus aureus was inhibited by butanol, aqueous extracts of Rosa damascena receptacles and butanol extract of Inula viscosa flowers (MIC of 500, 500, and 250 microg/mL) respectively. Rosa damascena receptacles and Verbascum sinaiticum flowers ethanol extract showed lowest cytotoxicity against Vero cell line (IC50 of 454.11 and 367.11). Most toxic was the ethanol extract of Ononis hirta aerial parts (IC50 72.50 microg/mL). Flavonoids and terpenoids were present in all plants. Ononis hirta and Narcissus tazetta contained alkaloids. The results validate the use of these plants and report for the first time bioactivity of Rosa damascena receptacles and further justifies the use of such screening programs in the quest for new drugs.
研究了 14 种植物的 51 种不同部位的提取物的抗菌活性和细胞毒性。用乙醇、甲醇、水、正丁醇和正己烷提取物对三种革兰氏阴性菌、两种革兰氏阳性菌和两种真菌进行了测试。用 MTT 和 TLC 测定法分别测定细胞毒性和植物化学筛选。在 51 种提取物中，有 22 种对不同微生物具有活性，MIC 范围为 62.5 至 1000μg/mL。对桃金娘科植物花托的丁醇提取物的活性最高（100%抑制），对鼠伤寒沙门氏菌和蜡状芽孢杆菌的 MIC 分别为 62.5 和 250μg/mL。水仙鳞茎的丁醇提取物和蔷薇科植物花托的水提取物对白色念珠菌均有活性（MIC 为 125μg/mL）。耐甲氧西林金黄色葡萄球菌被蔷薇科植物花托的丁醇、水提取物和毛蕊花的丁醇提取物抑制（MIC 为 500、500 和 250μg/mL）。蔷薇科植物花托和阿拉伯婆婆纳的乙醇提取物对非洲绿猴肾细胞系的细胞毒性最低（IC50 分别为 454.11 和 367.11）。毒性最强的是 Ononis hirta 地上部分的乙醇提取物（IC50 为 72.50μg/mL）。所有植物中均含有类黄酮和萜类化合物。Ononis hirta 和水仙鳞茎含有生物碱。研究结果验证了这些植物的使用，并首次报道了大马士革蔷薇花托的生物活性，进一步证明了此类筛选方案在寻找新药方面的应用。
