利用 CRISPR/Cas9 介导的抗病毒性在农业重要作物中的发展。
Development of CRISPR/Cas9 mediated virus resistance in agriculturally important crops.
机构信息
a Amity Institute of Biotechnology, Amity University Haryana , Gurgaon , India.
b Amity Institute of Microbial Technology, Amity University , Noida , India.
出版信息
Bioengineered. 2017 May 4;8(3):274-279. doi: 10.1080/21655979.2017.1297347.
Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) system of targeted genome editing has already revolutionized the plant science research. This is a RNA guided programmable endonuclease based system composed of 2 components, the Cas9 nuclease and an engineered guide RNA targeting any DNA sequence of the form N20-NGG for novel genome editing applications. The CRISPR/Cas9 technology of targeted genome editing has been recently applied for imparting virus resistance in plants. The robustness, wide adaptability, and easy engineering of this system has proved its potential as an antiviral tool for plants. Novel DNA free genome editing by using the preassembled Cas9/gRNA ribonucleoprotein complex for development of virus resistance in any plant species have been prospected for the future. Also, in this review we have discussed the reports of CRISPR/Cas9 mediated virus resistance strategy against geminiviruses by targeting the viral genome and transgene free strategy against RNA viruses by targeting the host plant factors. In conclusion, CRISPR/Cas9 technology will provide a more durable and broad spectrum viral resistance in agriculturally important crops which will eventually lead to public acceptance and commercialization in the near future.
成簇规律间隔短回文重复（CRISPR）/CRISPR 相关核酸酶 9（Cas9）系统的靶向基因组编辑已经彻底改变了植物科学研究。这是一种基于 RNA 指导的可编程内切酶系统，由 2 个组件组成，Cas9 核酸酶和一种针对任何 DNA 序列（形式为 N20-NGG）的工程靶向 RNA，用于新型基因组编辑应用。靶向基因组编辑的 CRISPR/Cas9 技术最近已被应用于赋予植物抗病毒能力。该系统的稳健性、广泛适应性和易于工程化已证明其具有作为植物抗病毒工具的潜力。未来有望通过使用预组装的 Cas9/gRNA 核糖核蛋白复合物进行无 DNA 的基因组编辑，从而在任何植物物种中开发抗病毒能力。此外，在这篇综述中，我们讨论了针对病毒基因组的 CRISPR/Cas9 介导的抗病毒策略以及针对宿主植物因子的无转基因 RNA 病毒的策略的报告。总之，CRISPR/Cas9 技术将为农业重要作物提供更持久和广谱的抗病毒能力，最终将在不久的将来获得公众的认可和商业化。
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