Hanafi Mervat Y, Zaher Eman L M, El-Adely Soha E M, Sakr Ahmed, Ghobashi Ahmed H M, Hemly Madiha H, Kazem Amani H, Kamel Maher A
Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria 21561, Egypt.
Department of Pharmacology, Medical Research Institute, Alexandria University, Alexandria 21561, Egypt.
Exp Ther Med. 2018 Jun;15(6):5091-5099. doi: 10.3892/etm.2018.6028. Epub 2018 Apr 3.
The present study was designed to investigate the therapeutic effects of bee venom (BV) on high-fat diet (HFD)-induced non-alcoholic fatty liver (NAFL) in rats at different levels. Histological manifestations, hepatic lipid content, liver function tests, glucose homeostasis, lipid abnormalities, adipocytokines, lipid peroxidation, disturbed glutathione and antioxidant enzymes systems and dysregulation of Nrf2 transcription factor were assessed. In the present study, the NAFL rats were subcutaneously treated with BV with different doses (0.01, 0.05, 0.1 mg/kg). The results indicated that BV treatment completely normalized the lipid profile values of NAFL rats. Fasting blood sugar, insulin level and homeostatic model assessment of insulin resistance significantly decreased. BV treated rats showed a significantly lower level of all liver enzymes and bilirubin. Moreover, BV treatment significantly increased the levels of active nuclear erythroid factor 2 like 2, glutathione (GSH) (total and reduced), GSH/glutathione disulphide ratio and activities of glutathione reductase, glutathione-S-transferase and glutathione peroxidase (total and Se-dependent). The level of tumor necrosis factor-α was reduced. Treatment showed correction of adiponectin level, and significant downregulation of hepatic triglycerides and cholesterol. At the histological level, BV improved the architecture of liver cells showing normal sinusoids. It may be concluded that BV may represent an interesting therapeutic alternative for the treatment of NAFL disease.
本研究旨在探讨蜂毒（BV）对不同程度高脂饮食（HFD）诱导的大鼠非酒精性脂肪肝（NAFL）的治疗效果。评估了组织学表现、肝脏脂质含量、肝功能测试、葡萄糖稳态、脂质异常、脂肪细胞因子、脂质过氧化、谷胱甘肽和抗氧化酶系统紊乱以及Nrf2转录因子失调情况。在本研究中，对NAFL大鼠皮下注射不同剂量（0.01、0.05、0.1mg/kg）的BV。结果表明，BV治疗使NAFL大鼠脂质谱值完全恢复正常。空腹血糖、胰岛素水平和胰岛素抵抗稳态模型评估值显著降低。BV治疗的大鼠所有肝酶和胆红素水平显著降低。此外，BV治疗显著提高了活性核红细胞因子2样2、谷胱甘肽（GSH）（总谷胱甘肽和还原型谷胱甘肽）、GSH/谷胱甘肽二硫化物比值以及谷胱甘肽还原酶、谷胱甘肽-S-转移酶和谷胱甘肽过氧化物酶（总酶和硒依赖性酶）的活性。肿瘤坏死因子-α水平降低。治疗显示脂联素水平得到纠正，肝脏甘油三酯和胆固醇显著下调。在组织学水平上，BV改善了肝细胞结构，显示出正常的肝血窦。可以得出结论，BV可能是治疗NAFL疾病的一种有趣的治疗选择。
