Department of Internal Medicine, Angiology and Physical Medicine, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Batorego Street 15, 41-902 Bytom, Poland.
Department of Medical Physics, Chelkowski Institute of Physics, University of Silesia, 4 Uniwersytecka St., 40-007 Katowice, Poland.
Oxid Med Cell Longev. 2018 May 2;2018:7365490. doi: 10.1155/2018/7365490. eCollection 2018.
The aim of the study was to estimate the impact of whole body cryotherapy (WBC) on oxidative stress when performed in a closed cryochamber on patients with ankylosing spondylitis (AS).
The effect of ten WBC procedures lasting 3 minutes a day with a subsequent 60-minute session kinesiotherapy on oxidative stress in male AS patients (WBC group = 16) was investigated. To assess the disease activity, the Bath Ankylosing Spondylitis Diseases Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) were calculated. The WBC group was compared to the kinesiotherapy only (KT; = 16) group. The routine parameters of oxidative stress (antioxidant enzymatic and nonenzymatic antioxidant status, lipid peroxidation products, total oxidative status (TOS), and oxidative stress index (OSI)) were estimated one day before the beginning and one day after the completion of the research program.
After the completion of the treatment in the WBC group, a significant decrease of oxidative stress markers (TOS and OSI) and a significant increase of total antioxidant status were observed. The erythrocyte activity of glutathione peroxidase, glutathione reductase decreased significantly in both groups, but the differences of activity of that enzymes prior to post treatment values (Δ) in the KT group were significantly higher as compared to the WBC group. The activity of erythrocyte catalase and plasma ZnCu isoenzyme of superoxide dismutase showed a decreased tendency; erythrocyte total superoxide dismutase activity showed an increased tendency in the WBC group after the completion of the treatment. The BASDAI and BASFI decreased significantly in both groups, but the differences of value indexes prior to post treatment (Δ) were significantly higher in the WBC than KT group.
WBC performed in a closed cryochamber decreases oxidative stress and improves BASDAI and BASFI indexes in male patients during the active phase of ankylosing spondylitis.
本研究旨在评估在封闭 cryochamber 中进行全身冷冻疗法（WBC）对强直性脊柱炎（AS）患者氧化应激的影响。
研究了 16 例男性 AS 患者每天进行 10 次 WBC 治疗，每次 3 分钟，随后进行 60 分钟的运动疗法，以评估氧化应激的影响。为了评估疾病活动度，计算了 Bath 强直性脊柱炎疾病活动指数（BASDAI）和 Bath 强直性脊柱炎功能指数（BASFI）。将 WBC 组与仅运动疗法（KT）组（n = 16）进行比较。在研究计划开始前一天和结束后一天，评估常规氧化应激参数（抗氧化酶和非酶抗氧化状态、脂质过氧化产物、总氧化状态（TOS）和氧化应激指数（OSI））。
在 WBC 组完成治疗后，观察到氧化应激标志物（TOS 和 OSI）显著降低，总抗氧化状态显著增加。两组的红细胞谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性均显著降低，但 KT 组治疗前后（Δ）的酶活性差异明显高于 WBC 组。红细胞过氧化氢酶和血浆 ZnCu 超氧化物歧化酶同工酶活性呈下降趋势；WBC 组治疗后红细胞总超氧化物歧化酶活性呈上升趋势。两组的 BASDAI 和 BASFI 均显著降低，但 WBC 组治疗前后（Δ）的差值明显高于 KT 组。
在封闭 cryochamber 中进行 WBC 可降低氧化应激，并在强直性脊柱炎活动期改善男性患者的 BASDAI 和 BASFI 指数。
