Campus de Santiago, Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal.
Unité de Recherche et Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physico-chimiques et Biologiques, Université des Frères Mentouri Constantine 1, Constantine, Algeria.
Molecules. 2018 May 27;23(6):1285. doi: 10.3390/molecules23061285.
The phenolic profile of L., a species used in Moroccan traditional medicine, is disclosed. To obtain that profile the species extract was analyzed by ultra-high-performance chromatography coupled to photodiode-array detection and electrospray ionization/ion trap mass spectrometry (UHPLC-DAD-ESI/MS). Twenty-five phenolic compounds were identified from which isoorientin and 4--caffeoylquinic acid can be highlighted because they are the major ones. The antioxidant activity was significantly controlled by the fraction type, with the -butanol fraction showing the highest antioxidant activity (FRS = 64.46 µg/mL in the DPPH assay, FRS = 27.87 µg/mL in the ABTS assay and EC = 161.11 µg/mL in the reducing power assay). A phytochemical study of the -butanol fraction was performed, and some important flavone glycosides were isolated. Among them the tamarixetin derivatives-the less common ones-can be emphasized. This phytochemical study and polyphenolic profile can be correlated with extracts in vitro antioxidant activity. Moreover, it can be regarded as an evidence of its medicinal use and can incentivize its consumption.
本文揭示了在摩洛哥传统医学中使用的物种 L. 的酚类成分。为了获得该成分图谱，采用超高效液相色谱-光电二极管阵列检测-电喷雾/离子阱质谱联用（UHPLC-DAD-ESI/MS）对该物种提取物进行了分析。从其中鉴定出 25 种酚类化合物，其中异荭草素和 4--咖啡酰奎宁酸尤为突出，因为它们是主要成分。抗氧化活性显著受馏分类型控制，其中正丁醇馏分表现出最高的抗氧化活性（DPPH 测定中 FRS 为 64.46 µg/mL，ABTS 测定中 FRS 为 27.87 µg/mL，还原力测定中 EC 为 161.11 µg/mL）。对正丁醇馏分进行了植物化学研究，分离出一些重要的黄酮糖苷。其中，塔玛瑞琴衍生物——不太常见的一类——可以被强调。这种植物化学研究和多酚成分图谱可以与提取物的体外抗氧化活性相关联。此外，它可以被视为其药用用途的证据，并鼓励其使用。
