Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami, Sagamihara, Kanagawa 252-0375, Japan.
Research and Development Department, HOYA Technosurgical Corporation, 1-1-110 Tsutsujigaoka, Akishima, Tokyo 196-0012, Japan.
Biomed Res Int. 2018 May 15;2018:4560647. doi: 10.1155/2018/4560647. eCollection 2018.
Calcium phosphate cement (CPC) has good release efficiency and has therefore been used as a drug delivery system for postoperative infection. The release profile of CPC has mainly been evaluated by studies, which are carried out by immersing test specimens in a relatively large amount of solvent. However, it remains unclear whether antibiotic-impregnated CPC has sufficient clinical effects and release . We examined the release profile of CPC impregnated with vancomycin (VCM) and compared this with that of polymethylmethacrylate (PMMA) cement. To evaluate the release profile , the test specimens were immersed in 10 mL sterile phosphate-buffered saline per gram of test specimen and incubated at 37°C for 56 days in triplicate. For experiments, the test specimens were implanted between the fascia and muscle of the femur of rats. Residual VCM was extracted from the removed test specimens to determine the amount of VCM released into rat tissues. CPC released more VCM over a longer duration than PMMA . Released levels of VCM from CPC/VCM were 3.4-fold, 5.0-fold, and 8.6-fold greater on days 1, 7, and 28, respectively, than those released on the corresponding days from PMMA/VCM and were drastically greater on day 56 due to inefficient release from PMMA/VCM. The amount of VCM released from CPC and PMMA was much higher than the minimum inhibitory concentration (1.56 g) and lower than the detection limit, respectively. Our findings suggest that CPC is a suitable material for releasing antibiotics for local action against established postoperative infection.
磷酸钙骨水泥（CPC）具有良好的释放效率，因此已被用作术后感染的药物输送系统。CPC 的释放特性主要通过研究来评估，这些研究通过将测试标本浸入相对大量的溶剂中来进行。然而，尚不清楚载抗生素 CPC 是否具有足够的临床效果和释放。我们检查了载万古霉素（VCM）的 CPC 的释放特性，并将其与聚甲基丙烯酸甲酯（PMMA）水泥进行了比较。为了评估释放特性，将测试标本浸入 10ml 无菌磷酸盐缓冲盐水（每克测试标本）中，并在 37°C 下孵育 56 天，一式三份。对于实验，将测试标本植入大鼠股骨的筋膜和肌肉之间。从取出的测试标本中提取残留的 VCM，以确定释放到大鼠组织中的 VCM 量。CPC 比 PMMA 释放更多的 VCM 持续时间更长。CPC/VCM 释放的 VCM 水平在第 1、7 和 28 天分别比 PMMA/VCM 释放的 VCM 水平高 3.4 倍、5.0 倍和 8.6 倍，在第 56 天由于 PMMA/VCM 释放效率低下，释放水平更高。CPC 和 PMMA 释放的 VCM 量均远高于最低抑菌浓度（1.56g），低于检测限。我们的研究结果表明，CPC 是一种适合局部释放抗生素以对抗已建立的术后感染的材料。
