Prosolov Konstantin A, Komarova Ekaterina G, Kazantseva Ekaterina A, Lozhkomoev Aleksandr S, Kazantsev Sergei O, Bakina Olga V, Mishina Marina V, Zima Anastasia P, Krivoshchekov Sergei V, Khlusov Igor A, Sharkeev Yurii P
Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, Siberian Branch of Russian Academy of Sciences, 634055 Tomsk, Russia.
Department of Strength and Design, National Research Tomsk State University, 634030 Tomsk, Russia.
Materials (Basel). 2022 Jul 1;15(13):4643. doi: 10.3390/ma15134643.
Drug delivery systems based on calcium phosphate (CaP) coatings have been recently recognized as beneficial drug delivery systems in complex cases of bone diseases for admission of drugs in the localized area, simultaneously inducing osteoinduction because of the bioavailable Ca and P ions. However, micro-arc oxidation (MAO) deposition of CaP does not allow for the formation of a coating with sufficient interconnected porosity for drug delivery purposes. Here, we report on the method to deposit CaP-based coatings using a new hybrid ultrasound-assisted MAO (UMAOH) method for deposition of coatings for drug delivery that could carry various types of drugs, such as cytostatic, antibacterial, or immunomodulatory compositions. Application of UMAOH resulted in coatings with an Ra roughness equal to 3.5 µm, a thickness of 50-55 µm, and a combination of high values of internal and surface porosity, 39 and 28%, respectively. The coating is represented by the monetite phase that is distributed in the matrix of amorphous CaP. Optimal conditions of coating deposition have been determined and used for drug delivery by impregnation with Vancomycin, 5-Fluorouracil, and Interferon-α-2b. Cytotoxicity and antimicrobial activity of the manufactured drug-carrying coatings have been studied using the three different cell lines and methicillin-resistant .
基于磷酸钙（CaP）涂层的药物递送系统最近被认为是治疗复杂骨病的有益药物递送系统，可将药物输送至局部区域，同时由于生物可利用的钙和磷离子而诱导骨诱导。然而，CaP的微弧氧化（MAO）沉积无法形成具有足够相互连通孔隙率的涂层以用于药物递送目的。在此，我们报道了一种使用新型混合超声辅助MAO（UMAOH）方法沉积基于CaP的涂层的方法，该方法用于沉积可携带各种类型药物（如细胞抑制、抗菌或免疫调节组合物）的药物递送涂层。UMAOH的应用导致涂层的粗糙度Ra等于3.5 µm，厚度为50 - 55 µm，内部孔隙率和表面孔隙率分别高达39%和28%。该涂层由分布在无定形CaP基质中的磷酸二氢钙相组成。已确定涂层沉积的最佳条件，并通过用万古霉素、5 - 氟尿嘧啶和干扰素 - α - 2b浸渍用于药物递送。使用三种不同的细胞系和耐甲氧西林的[此处原文不完整]研究了制造的载药涂层的细胞毒性和抗菌活性。
