Harmenberg J, Wahren B, Sundqvist V A, Levén B
J Antimicrob Chemother. 1985 May;15(5):567-73. doi: 10.1093/jac/15.5.567.
An immunoassay that enables one to assess both viral multiplicity and sensitivity to antiviral drugs, was used to determine the sensitivity of untreated patients' virus isolates. The upper border levels for judging clinical isolates sensitive to Ara-A, ACV, PFA or IDU were calculated from a total of 48 primary herpes simplex isolates. Although five isolates were considered less sensitive to one drug and one isolate to two drugs, all were sensitive at a lower virus multiplicity. Analysis of these isolates showed that no isolate was genetically resistant, but that multiplicity dependence of drugs was high with ACV and Ara-A, lower with PFA. In view of the multiplicity dependence of HSV sensitivity to different drugs, it is recommended that isolates from treatment failures or isolates considered resistant should be assayed in detail.
一种能够评估病毒感染复数和对抗病毒药物敏感性的免疫测定法，被用于测定未经治疗患者的病毒分离株的敏感性。根据总共48株原发性单纯疱疹病毒分离株，计算出判断对阿糖腺苷、阿昔洛韦、磷甲酸或茚地那韦敏感的临床分离株的上限水平。尽管有5株分离株被认为对一种药物敏感性较低，1株分离株对两种药物敏感性较低，但在较低的病毒感染复数下所有分离株均敏感。对这些分离株的分析表明，没有分离株具有基因抗性，但阿昔洛韦和阿糖腺苷的药物感染复数依赖性较高，磷甲酸的较低。鉴于单纯疱疹病毒对不同药物的敏感性存在感染复数依赖性，建议对治疗失败的分离株或被认为耐药的分离株进行详细检测。
