Rai Nitish, Venugopalan G, Pradhan Rashmita, Ambastha Akash, Upadhyay Ashish Datt, Dwivedi Sadanand, Dey Aparajit B, Dey Sharmistha
1Department of Biophysics.
2Department of Geriatric Medicine.
Aging Dis. 2018 Apr 1;9(2):220-227. doi: 10.14336/AD.2017.0423. eCollection 2018 Apr.
Frailty in elderly is very much familiar with a decline in the musculoskeletal system. Muscle degeneration in the lower organism was observed due to loss of anti-oxidant protein Sestrin. The aim of the study is to determine the level of Sestrin1 and Sestrin2 in the serum of frail and non-frail elderly to associate their impact in frailty syndrome. Subjects with age ≥ 65 years were enrolled from Geriatric Medicine OPD of All India Institute of Medical Sciences, New Delhi (N= 92). Among them, 51 subjects were identified as frail and rest 41 were regarded as non-frail according to "deficit accumulation model of Rockwood." The study was performed by surface plasmon resonance and validated by western blot. Sestrin1 and Sestrin2 were found to be significantly reduced in frail compare to non-frail elderly. Furthermore, even after the adjustment for age, gender and education, the level of Sestrin1 and Sestrin2 remain significantly lower across the groups. The Sestrin1 level was significantly lower in various categories like age, gender, BMI, education, ADL, number of co-morbidity along with other clinico-pathological features. ROC analysis also revealed the distinction of frail and non-frail in respect to serum Sestrin1 and Sestrin2. This study highlighted the new and promising role of serum Sestrin in frail and non-frail elderly. In future, it can be utilized as molecular marker to assess the potential diagnostic value for clinical purpose.
老年人的衰弱与肌肉骨骼系统的衰退密切相关。由于抗氧化蛋白Sestrin的缺失，观察到低等生物中的肌肉退化。本研究的目的是确定衰弱和非衰弱老年人血清中Sestrin1和Sestrin2的水平，以关联它们对衰弱综合征的影响。年龄≥65岁的受试者来自新德里全印度医学科学研究所老年医学门诊（N = 92）。其中，根据“Rockwood的缺陷累积模型”，51名受试者被确定为衰弱，其余41名被视为非衰弱。该研究通过表面等离子体共振进行，并通过蛋白质印迹法进行验证。与非衰弱老年人相比，发现衰弱老年人的Sestrin1和Sestrin2显著降低。此外，即使在对年龄、性别和教育程度进行调整后，各组中Sestrin1和Sestrin2的水平仍然显著较低。在年龄、性别、体重指数、教育程度、日常生活活动能力、合并症数量以及其他临床病理特征等各类别中，Sestrin1水平均显著较低。ROC分析还揭示了血清Sestrin1和Sestrin2在区分衰弱和非衰弱方面的作用。本研究突出了血清Sestrin在衰弱和非衰弱老年人中具有新的且有前景的作用。未来，它可作为分子标志物用于评估临床目的的潜在诊断价值。
