Jiang Lihong, Zhao Jiarun, Lian Jiazhang, Xu Zhinan
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Synth Syst Biotechnol. 2018 Feb 22;3(2):90-96. doi: 10.1016/j.synbio.2018.02.003. eCollection 2018 Jun.
Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal production represents a grand challenge and an unavoidable barrier for metabolic engineering. Recently, cell-free protein synthesis system (CFPS) has been emerging as an enabling alternative to address challenges in biomanufacturing. This review summarizes the recent progresses of CFPS in rapid prototyping of biosynthetic pathways and genetic circuits (biosensors) to speed up design-build-test (DBT) cycles of metabolic engineering and synthetic biology.
在过去十年中，代谢工程和合成生物学的进展推动了利用微生物细胞工厂生产许多高附加值化合物和商品化学品。然而，由于细胞代谢的复杂性，优化代谢途径以实现最大产量是代谢工程面临的巨大挑战和不可避免的障碍。最近，无细胞蛋白质合成系统（CFPS）已成为应对生物制造挑战的一种可行替代方案。本文综述了CFPS在生物合成途径和遗传电路（生物传感器）快速原型设计方面的最新进展，以加速代谢工程和合成生物学的设计-构建-测试（DBT）循环。
