Wang Tian-Shu, Xie Jiu-Yan, Wang Li-Ying, Chen San-Feng
State Key Laboratory for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Curr Microbiol. 2018 Oct;75(10):1267-1273. doi: 10.1007/s00284-018-1519-8. Epub 2018 Jun 8.
A novel bacterium SX-49 with nitrogen-fixing capability was isolated from the rhizosphere soil of maize. Phylogenetic analysis of nifH gene fragment and 16S rRNA gene sequence revealed that the strain SX-49 is a member of the genus Paenibacillus. Values of 16S rRNA gene sequence similarity were highest between SX-49 and P. jamilae DSM 13815 (97.0%), P. brasiliensis DSM 14914 (97.8%), P. polymyxa DSM 36 (97.5%), and P. terrae DSM 15891 (98.8%). The similarity between SX-49 and other Paenibacillus species was < 97.0%. DNA-DNA hybridization values between strain SX-49 and the four type strains were P. jamilae DSM 13815: 40.6%, P. brasiliensis DSM 14914: 27.9%, P. polymyxa DSM 36: 29.2%, and P. terrae DSM 15891: 66.4%. The DNA G+C content of SX-49 was 46.4 mol%. The predominant fatty acids were anteiso-C, C and iso-C. The predominant isoprenoid quinone was MK-7. The genome contains 5628 putative protein-coding sequences (CDS), 6 rRNAs and 56 tRNAs. The phenotypic and genotypic characteristics, DNA-DNA relatedness, and genome features suggest that SX-49 represents a novel species of the genus Paenibacillus, and the name Paenibacillus maysiensis sp. nov. is proposed.
从玉米根际土壤中分离出一种具有固氮能力的新型细菌SX-49。对nifH基因片段和16S rRNA基因序列的系统发育分析表明，菌株SX-49是芽孢杆菌属的成员。SX-49与贾米拉芽孢杆菌DSM 13815（97.0%）、巴西芽孢杆菌DSM 14914（97.8%）、多粘芽孢杆菌DSM 36（97.5%）和土壤芽孢杆菌DSM 15891（98.8%）之间的16S rRNA基因序列相似性值最高。SX-49与其他芽孢杆菌属物种之间的相似性<97.0%。菌株SX-49与四种模式菌株之间的DNA-DNA杂交值分别为：贾米拉芽孢杆菌DSM 13815：40.6%，巴西芽孢杆菌DSM 14914：27.9%，多粘芽孢杆菌DSM 36：29.2%，土壤芽孢杆菌DSM 15891：66.4%。SX-49的DNA G+C含量为46.4 mol%。主要脂肪酸为anteiso-C、C和iso-C。主要类异戊二烯醌为MK-7。该基因组包含5628个推定的蛋白质编码序列（CDS）、6个rRNA和56个tRNA。表型和基因型特征、DNA-DNA相关性以及基因组特征表明，SX-49代表芽孢杆菌属的一个新物种，因此提出了新名称玉米芽孢杆菌（Paenibacillus maysiensis sp. nov.）。
