Department of Dermatology & Venereology, All India Institute of Medical Sciences, New Delhi, India.
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Indian J Med Res. 2018 Apr;147(4):384-390. doi: 10.4103/ijmr.IJMR_657_16.
BACKGROUND & OBJECTIVES: Vitiligo is an acquired skin disease characterized by depigmented areas of the skin. Increased release of catecholamines from autonomic nerve endings in microenvironment of melanocytes in affected skin might be involved in the aetiopathogenesis of vitiligo. Levels of catecholamines are considered as being related to onset or worsening of the disease. Therefore, in this study, the role of catecholamines was evaluated in mapping disease stability and outcome of vitiligo patients undergoing melanocyte transfer.
In this study, circulatory and urinary levels of catecholamine (CA) and vanillylmandelic acid (VMA) were determined in 45 individuals (30 vitiligo patients and 15 healthy controls) using ELISA.
A significant increase for plasma and urinary catecholamines along with VMA was observed as compared to healthy controls. When the pre- and post-intervention levels were analyzed in responders and non-responders, respectively, only dopamine showed significant decline in urine, rest of the molecules in plasma as well as urine showed non-significant decline except VMA which showed insignificant increase.
INTERPRETATION & CONCLUSIONS: Levels of plasma/urinary epinephrine, and plasma dopamine, could not be established as biomarkers for disease stability or successful outcome of autologous melanocyte transfer in generalized vitiligo patients. However, dopamine (urine) might be of help in determining the stability in patients with generalized vitiligo undergoing melanocyte transfer. Further studies need to be done on a large sample of patients to confirm our findings.
白癜风是一种后天性皮肤疾病，其特征是皮肤出现色素脱失区域。受影响皮肤中黑素细胞微环境中自主神经末梢释放的儿茶酚胺可能参与了白癜风的发病机制。儿茶酚胺的水平被认为与疾病的发作或恶化有关。因此，在这项研究中，我们评估了儿茶酚胺在绘制接受黑素细胞移植的白癜风患者疾病稳定性和结局中的作用。
本研究采用 ELISA 法检测了 45 名个体（30 名白癜风患者和 15 名健康对照者）的循环和尿儿茶酚胺（CA）和香草扁桃酸（VMA）水平。
与健康对照组相比，血浆和尿液中的儿茶酚胺和 VMA 水平显著升高。在分析有反应者和无反应者的治疗前后水平时，只有尿多巴胺显著下降，血浆和尿液中的其余分子均无显著下降，除 VMA 外，其无显著增加。
血浆/尿液中的肾上腺素、去甲肾上腺素和多巴胺水平不能作为白癜风患者疾病稳定性或自体黑素细胞移植成功结局的生物标志物。然而，多巴胺（尿液）可能有助于确定接受黑素细胞移植的泛发性白癜风患者的稳定性。需要对大量患者进行进一步研究以证实我们的发现。
