Laboratory of Animal Physiology, Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Viopolis, Larissa, Greece.
Laboratory of Exercise Biochemistry, Exercise Physiology and Sports Nutrition (SmArT Lab), Department of Physical Education and Sport Science, University of Thessaly, 42100 Trikala, Greece.
Oxid Med Cell Longev. 2018 Jul 17;2018:6857190. doi: 10.1155/2018/6857190. eCollection 2018.
It has been proposed that exercise-induced oxidative stress and adaptations are dependent on training status. In this study, we examined the effects of training background on free radical generation and adaptations after eccentric exercise. Forty volunteers were divided into two groups (trained and untrained) and were asked to perform eccentric exercise. Then, their blood samples were collected pre, 24, 48, and 72 hours postexercise. Biomarkers indicating oxidative damage and the antioxidant profiles of the participants were measured in plasma and erythrocyte lysate both spectrophotometrically and chromatographically. The results revealed that the untrained group depicted more severe oxidative damage (protein carbonyls, malondialdehyde), weaker antioxidant status (reduced glutathione, static and capacity oxidation-reduction potential), and weaker radical-scavenging activity (superoxide radical scavenging and reducing power) compared to the trained participants. Our findings show that trained individuals are less susceptible to oxidative damage and suggest that generalized nutritional recommendations regarding recovery after exercise should be avoided.
有人提出，运动引起的氧化应激和适应取决于训练状态。在这项研究中，我们研究了训练背景对离心运动后自由基生成和适应的影响。40 名志愿者被分为两组（训练有素和未训练），并要求进行离心运动。然后，在运动前、24 小时、48 小时和 72 小时采集他们的血液样本。使用分光光度法和色谱法在血浆和红细胞裂解物中测量表明氧化损伤的生物标志物和参与者的抗氧化特性。结果表明，与训练有素的参与者相比，未训练组表现出更严重的氧化损伤（蛋白质羰基、丙二醛）、较弱的抗氧化状态（还原型谷胱甘肽、静态和容量氧化还原电势）和较弱的自由基清除活性（超氧自由基清除和还原能力）。我们的研究结果表明，训练有素的个体不易受到氧化损伤的影响，并表明运动后恢复的一般性营养建议应该避免。
