School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Biosens Bioelectron. 2018 Nov 15;119:156-162. doi: 10.1016/j.bios.2018.07.060. Epub 2018 Jul 30.
Early diagnosis of cancers is critical for prevention of metastasis and early treatment. The study reports an electrochemical sensor for detection of cancer cell based on folic acid (FA) and octadecylamine (OA)-functionalized graphene aerogel microspheres (FA-GAM-OA). Citric acid was mixed with FA and OA and heated at 180 °C for 4 h to form FA and OA-functionalized graphene oxide. The graphene oxide was employed as solid particle surfactant for stabilizing toluene-in-water emulsion. The graphene oxide sheets in the emulsion were self-assembled into graphene oxide gel microspheres on the water/toluene interfaces. Followed by free drying and reduction in H at 400 °C for 5 h. The resulted FA-GAM-OA shows a sphere-like structure with an average diameter of 1.2 µm, the rich of open-pores and folic acid groups. Small particle size and good hydrophilicity make FA-GAM-OA can be dispersed in water for sensor preparation. The small size of graphene sheets and their self-assembly avoid a serious agglomeration of graphene sheets. The FA-GAM-OA offers a large surface area (1723.6 m g) and high electronic conductivity (2978.2 S m). The covalent linkage and ordered alignment of folic acid groups at FA-GAM-OA surface achieve to specific cancer cell capture with high capture efficiency. The electrochemical sensor based on FA-GAM-OA exhibits extremely good analytical performances in detection of liver cancer cells with a linear range of 5-10 cell mL giving a low detection limit of 5 cells mL (S/N = 3). The method was successfully applied to electrochemical detection of liver cancer cells in whole blood.
早期癌症诊断对于预防转移和早期治疗至关重要。本研究报告了一种基于叶酸（FA）和十八胺（OA）功能化石墨烯气凝胶微球（FA-GAM-OA）的用于检测癌细胞的电化学传感器。柠檬酸与 FA 和 OA 混合并在 180°C 下加热 4 h 以形成 FA 和 OA 功能化氧化石墨烯。氧化石墨烯被用作甲苯/水乳液的固体颗粒表面活性剂。乳液中的氧化石墨烯片在水/甲苯界面上自组装成氧化石墨烯凝胶微球。然后在 H 中于 400°C 下自由干燥并还原 5 h。所得的 FA-GAM-OA 呈球形结构，平均直径为 1.2 µm，具有丰富的开放孔和叶酸基团。小颗粒尺寸和良好的亲水性使 FA-GAM-OA 能够分散在水中用于传感器制备。石墨烯片的小尺寸及其自组装避免了石墨烯片的严重团聚。FA-GAM-OA 提供了大的表面积（1723.6 m g）和高的电子电导率（2978.2 S m）。FA-GAM-OA 表面叶酸基团的共价键合和有序排列实现了对特定癌细胞的高捕获效率的捕获。基于 FA-GAM-OA 的电化学传感器在检测肝癌细胞方面表现出极好的分析性能，线性范围为 5-10 个细胞 mL，检测限低至 5 个细胞 mL（S/N=3）。该方法成功地应用于全血中肝癌细胞的电化学检测。
