Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, India.
Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur, India.
Carbohydr Polym. 2018 Nov 15;200:572-582. doi: 10.1016/j.carbpol.2018.08.030. Epub 2018 Aug 9.
Chronic wounds suffer impaired healing that results in psychological effect and morbity in addition to economic burden on the patient. These wounds are characterized by prolonged inflammatory phase, poor collagen production, hampered angiogenesis, upregulation in reactive oxygen species (ROS) and matrix metalloproteases (MMP). In the current work, three different concentrations (03:01, 05:01 and 10:01) of flexible agar-sericin-glycerol hydrogel films with sericin as minor component were fabricated to improve collagen production and capture free radical species to expediate chronic wound healing. The films exhibited improved collagen production with a p-value ≤ 0.001 for all the three concentrations. With regards to free radical scavenging, the 03:01 and 05:01 films exhibited improved performance with a p-value ≤ 0.01, while, 10:01 film showed improvement with a p-value ≤ 0.05. This was confirmed by Sirius red staining and 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay. In addition, these films showed an adequate tensile strength of around 35 MPa with elongation at break higher than 25%. The as cast films exhibited satisfactory swelling behavior of around 320% in simulated wound fluid (SWF).
慢性伤口愈合受损，除了给患者带来经济负担外，还会导致心理影响和发病率。这些伤口的特点是炎症期延长、胶原蛋白生成不良、血管生成受阻、活性氧（ROS）和基质金属蛋白酶（MMP）上调。在目前的工作中，我们制备了三种不同浓度（03:01、05:01 和 10:01）的柔性琼脂丝胶-甘油水凝胶薄膜，其中丝胶作为少量成分，以提高胶原蛋白的生成并捕获自由基，从而加速慢性伤口愈合。所有三种浓度的薄膜都表现出明显改善的胶原蛋白生成，p 值均 ≤ 0.001。至于自由基清除，03:01 和 05:01 薄膜的性能有明显改善，p 值均 ≤ 0.01，而 10:01 薄膜的改善效果 p 值为 ≤ 0.05。这一点通过 Sirius 红染色和 2,2-二苯基-1-苦肼基（DPPH）试验得到了证实。此外，这些薄膜的拉伸强度约为 35 MPa，断裂伸长率高于 25%。铸膜在模拟伤口液（SWF）中表现出令人满意的溶胀行为，约为 320%。
