Diaz-Aroca E, Mendiola M V, Zabala J C, de la Cruz F
J Bacteriol. 1987 Jan;169(1):442-3. doi: 10.1128/jb.169.1.442-443.1987.
We determined the DNA sequences surrounding the junctions of IS91 in two insertion derivatives: pSU234 (pACYC184::IS91) and pSU240 (pBR322::IS91). The termini of IS91 consist of two imperfect inverted repeats eight base pairs long. Their sequence is 5'-TCGAGTAGG. . . CCTATCGA-3'. Insertion of IS91 did not generate direct repetitions in the target DNAs.
我们测定了两个插入衍生物中IS91连接处周围的DNA序列:pSU234(pACYC184::IS91)和pSU240(pBR322::IS91)。IS91的末端由两个长度为8个碱基对的不完全反向重复序列组成。它们的序列是5'-TCGAGTAGG...CCTATCGA-3'。IS91的插入在靶DNA中未产生直接重复序列。