ETH Zurich, Department of Health Sciences and Technology, Institute of Food, Nutrition and Health, IFNH, Sustainable Food Processing Laboratory, Schmelzbergstrasse 9, Zurich 8092, Switzerland.
Karlsruhe Institute of Technology, KIT, Institute for Pulsed Power and Microwave Technology, IHM, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany.
Bioresour Technol. 2019 Jan;271:402-408. doi: 10.1016/j.biortech.2018.09.124. Epub 2018 Sep 26.
Photoautotrophic microalgae based biorefinery concepts are currently not competitive compared to other established production systems. Therefore, innovative upstream processes need to be developed to increase the competitiveness of photoautotrophic microalgae biorefinery concepts. Abiotic sub-lethal stress induction via nanosecond pulsed electric field (nsPEF) treatment might be a viable process to increase the efficiency of photoautotrophic microalgae cultivation. In this work, an increased cell growth after nsPEF treatment was observable. Application of nsPEF to highly proliferating cells in a repetitive process resulted in a statistical significant increase in cell growth (p = 0.009). The effect was most pronounced after five days wherefore cellular structures and processes were analyzed to reveal a possible mechanism. Within this work, a protocol for increased cell proliferation with a possible mechanism was derived, which improves competitiveness of photoautotrophic microalgae biorefineries in the future. However, based on the derived mechanism, the results are also relevant for other microorganisms.
基于光自养微藻的生物炼制概念目前与其他已建立的生产系统相比没有竞争力。因此，需要开发创新的上游工艺，以提高光自养微藻生物炼制概念的竞争力。非生物亚致死胁迫诱导通过纳秒脉冲电场（nsPEF）处理可能是一种提高光自养微藻培养效率的可行方法。在这项工作中，观察到 nsPEF 处理后细胞生长增加。在重复过程中将 nsPEF 应用于增殖中的细胞会导致细胞生长的统计学显著增加（p=0.009）。在五天后效果最为明显，因此分析了细胞结构和过程以揭示可能的机制。在这项工作中，提出了一种具有潜在机制的增加细胞增殖的方案，这将提高未来光自养微藻生物炼制的竞争力。然而，根据推导的机制，这些结果也与其他微生物相关。
