Meis J M, Ordóñez N G, Gallager H S
Virchows Arch A Pathol Anat Histopathol. 1987;410(5):415-21. doi: 10.1007/BF00712761.
Six cases of sarcomatoid carcinoma of the breast (SCB) were studied with a panel of antibodies directed against epithelial and sarcomatoid components. The monoclonal antibodies (MoAb) AE-1/3, CAM 5.2, and CEA were used to detect epithelial differentiation; polyclonal antibodies against S-100 protein and MoAb against the intermediate filaments desmin and vimentin were used to detect mesenchymal differentiation in the sarcomatoid component. Six cases of invasive duct carcinoma (IDC) and two cases of cystosarcoma phyllodes (CP) were compared to SCB using the same panel of antibodies. In all three groups studied, the epithelial component in the majority of cases stained with anti-cytokeratin antibodies. S-100 protein antibodies stained the epithelial and sarcomatoid components in four cases of SCB; vimentin MoAb stained the epithelium in two cases and the sarcomatoid component in four cases of SCB, while MoAb CEA failed to stain any component of SCB. In contrast, the epithelium in five of six cases of IDC stained with CEA MoAb and only one of six stained for S-100 protein. Possible reasons for the discrepant immunohistochemical staining patterns among SCB, IDC and CP are discussed, in addition to the limitations and pitfalls of immunohistochemistry in diagnostic surgical pathology.
应用一组针对上皮和肉瘤样成分的抗体，对6例乳腺肉瘤样癌（SCB）进行了研究。单克隆抗体（MoAb）AE-1/3、CAM 5.2和癌胚抗原（CEA）用于检测上皮分化；针对S-100蛋白的多克隆抗体以及针对中间丝结蛋白和波形蛋白的MoAb用于检测肉瘤样成分中的间叶分化。使用同一组抗体，将6例浸润性导管癌（IDC）和2例叶状囊肉瘤（CP）与SCB进行比较。在所有研究的三组中，大多数病例的上皮成分均被抗细胞角蛋白抗体染色。S-100蛋白抗体在4例SCB中使上皮和肉瘤样成分染色；波形蛋白MoAb在2例SCB中使上皮染色，在4例SCB中使肉瘤样成分染色，而CEA MoAb未使SCB的任何成分染色。相比之下，6例IDC中有5例的上皮被CEA MoAb染色，6例中只有1例被S-100蛋白染色。除了免疫组织化学在诊断性外科病理学中的局限性和陷阱外，还讨论了SCB、IDC和CP之间免疫组织化学染色模式差异的可能原因。
