Liu Yanhong, O'Brien Jacqueline L, Ajami Nadim J, Scheurer Michael E, Amirian E Susan, Armstrong Georgina, Tsavachidis Spiridon, Thrift Aaron P, Jiao Li, Wong Matthew C, Smith Daniel P, Spitz Margaret R, Bondy Melissa L, Petrosino Joseph F, Kheradmand Farrah
Dan L. Duncan Comprehensive Cancer Center, Baylor College of Medicine Houston, TX 77030, USA.
Department of Medicine, Baylor College of Medicine Houston, TX 77030, USA.
Am J Cancer Res. 2018 Sep 1;8(9):1775-1787. eCollection 2018.
The composition and structure of site-specific microbiota have been investigated as potential biomarkers for a variety of chronic inflammatory diseases and cancers. While many studies have focused on the changes in the airway microbiota using respiratory specimens from patients with various respiratory diseases, more research is needed to explore the microbial profiles within the distal lung parenchyma in smokers with lung cancer and/or emphysema.
To describe and contrast lung tissue-associated microbial signatures in smokers with lung cancer and/or emphysema, we employed culture-independent pyrosequencing of 16S rRNA gene hypervariable V4 region and compositional analysis in non-malignant lung tissue samples obtained from 40 heavy smokers, including 10 emphysema-only, 11 lung cancer-only, and 19 with both lung cancer and emphysema.
The emphysema-only group presented a lower bacterial community evenness defined by a significantly lower Shannon diversity index compared to the lung cancer patients with or without emphysema ( = 0.006). Furthermore, community compositions of lung cancer patients with or without emphysema were characterized by a significantly lower abundance of Proteobacteria (primary the genera and ) and higher prevalence of Firmicutes () and Bacteroidetes (), compared to emphysema-only patients. In conclusion, the lung microbial composition and communities structures of smokers with lung cancer are distinct from the emphysema-only patients. Although preliminary, our findings suggest that lung microbiome changes could be a biomarker of lung cancer that could eventually be used to help screening for the disease.
特定部位微生物群的组成和结构已被作为多种慢性炎症性疾病和癌症的潜在生物标志物进行研究。虽然许多研究聚焦于利用各种呼吸道疾病患者的呼吸道标本分析气道微生物群的变化，但仍需要更多研究来探索肺癌和/或肺气肿吸烟者远侧肺实质内的微生物谱。
为了描述和对比肺癌和/或肺气肿吸烟者肺组织相关的微生物特征，我们对从40名重度吸烟者获取的非恶性肺组织样本进行了16S rRNA基因高变V4区的非培养焦磷酸测序和成分分析，这些吸烟者包括10名单纯肺气肿患者、11名单纯肺癌患者以及19名同时患有肺癌和肺气肿的患者。
与患有或未患肺气肿的肺癌患者相比，单纯肺气肿组细菌群落的均匀度较低，香农多样性指数显著更低（P = 0.006）。此外，与单纯肺气肿患者相比，患有或未患肺气肿的肺癌患者群落组成的特征是变形菌门（主要是属和属）丰度显著更低，而厚壁菌门（属）和拟杆菌门（属）的患病率更高。总之，肺癌吸烟者的肺微生物组成和群落结构与单纯肺气肿患者不同。尽管是初步研究，但我们的发现表明肺微生物组变化可能是肺癌的一种生物标志物，最终可用于帮助该疾病的筛查。
