O'Brien Claire L, Kiely Christopher J, Pavli Paul
1Medical School, Australian National University, Canberra, ACT 2600 Australia.
2Gastroenterology and Hepatology Unit, Canberra Hospital, Lvl 5, Bldg 10, Canberra Hospital, Yamba Drive, Garran, Canberra, ACT 2605 Australia.
Gut Pathog. 2018 Oct 10;10:44. doi: 10.1186/s13099-018-0265-6. eCollection 2018.
Reduced intestinal microbial diversity and bacterial imbalance (dysbiosis) are seen in studies of Crohn's disease. As it is difficult to obtain biopsy samples before disease presentation, the earliest mucosal lesions in Crohn's disease, aphthous ulcers, present the best chance at assessing microbial communities at the onset of disease or a new flare. The aim of our study was to compare the microbial communities of aphthous ulcers and adjacent normal mucosa from patients with Crohn's disease with normal mucosa from controls.
We did not observe bacterial imbalance or reduced alpha diversity in Crohn's disease aphthous ulcers and adjacent mucosa, relative to control biopsies. were common to all Crohn's disease and control samples, and there were no bacterial taxa unique to aphthous ulcers. The relative abundance of was not reduced in aphthous ulcers relative to control mucosa, and was not more likely to be detected in control samples.
In contrast to well-documented changes seen in late-stage Crohn's disease, microbial communities of aphthous ulcers do not display evidence of bacterial imbalance or reduced diversity. Our data suggest that dysbiosis occurs during active disease, and improves when patients are in remission.
在克罗恩病的研究中发现肠道微生物多样性降低和细菌失衡（生态失调）。由于在疾病表现出来之前很难获取活检样本，克罗恩病最早的黏膜病变即阿弗他溃疡，为评估疾病发作或新的发作期的微生物群落提供了最佳机会。我们研究的目的是比较克罗恩病患者阿弗他溃疡和相邻正常黏膜的微生物群落与对照组正常黏膜的微生物群落。
相对于对照活检样本，我们未观察到克罗恩病阿弗他溃疡和相邻黏膜存在细菌失衡或α多样性降低的情况。所有克罗恩病和对照样本中均常见，且没有阿弗他溃疡特有的细菌分类群。相对于对照黏膜，阿弗他溃疡中 的相对丰度没有降低，且在对照样本中被检测到的可能性也没有更高。
与晚期克罗恩病中记录充分的变化相反，阿弗他溃疡的微生物群落未显示出细菌失衡或多样性降低的证据。我们的数据表明，生态失调发生在疾病活动期，当患者缓解时会改善。
