Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China.
Sci Rep. 2018 Oct 23;8(1):15634. doi: 10.1038/s41598-018-34127-x.
Agrilus mali (Coleoptera: Buprestidae) is an invasive wood borer pest that has caused considerable damage to the Xinjiang wild fruit forest. In this study, we investigated the bacterial and fungal intestinal microbial communities of A. mali during different developmental stages, including larvae, pupae and newly eclosed adults or fed different diets (leaves of Malus halliana and Malus pumila) using Illumina MiSeq high-throughput sequencing technology. The results showed that microbial alpha diversity first increased and then decreased during the developmental stages, with the most dominant bacteria and fungi exhibiting the dynamic patterns "Decrease", "Increase" and "Fluctuation". With respect to the different diets, the bacterial communities were similar between the newly eclosed adults and adults fed M. pumila leaves, while the structure of the fungal communities showed great differences between newly eclosed adults and adults fed different diets. Through a co-correlation network analysis, we observed complex microbial interactions among bacterial and fungal taxa that were associated with potential diverse functions and intricate biological processes in the intestinal microbiota of A. mali. Overall, the results of this study demonstrated that the invasive insect A. mali harbours diverse, dynamic, and presumably multifunctional microbial communities, an understanding of which could improve our ability to develop more effective management approaches to control A. mali.
苹果小吉丁虫（鞘翅目：吉丁科）是一种入侵性蛀干害虫，对新疆野生果林造成了严重的破坏。在本研究中，我们利用 Illumina MiSeq 高通量测序技术，研究了苹果小吉丁虫在不同发育阶段（幼虫、蛹和刚羽化的成虫）以及取食不同食物（山荆子和苹果叶片）时的肠道细菌和真菌微生物群落。结果表明，在发育阶段，微生物α多样性先增加后减少，优势细菌和真菌表现出“减少”、“增加”和“波动”的动态模式。不同食物之间，刚羽化的成虫和取食苹果叶片的成虫的细菌群落相似，而真菌群落结构在刚羽化的成虫和取食不同食物之间表现出很大的差异。通过共相关网络分析，我们观察到与肠道微生物群中潜在多样功能和复杂生物过程相关的细菌和真菌类群之间复杂的微生物相互作用。总的来说，本研究的结果表明，入侵昆虫苹果小吉丁虫体内蕴藏着多样、动态且可能多功能的微生物群落，这有助于提高我们开发更有效的管理方法来控制苹果小吉丁虫的能力。
