Al-Marzooq Farah, Bayat Shahad Al, Sayyar Farah, Ishaq Hamdah, Nasralla Husain, Koutaich Rayan, Kawas Sausan Al
Sharjah Institute for Medical Research, University of Sharjah, Sharjah, UAE.
Department of Oral and Craniofacial Health, College of Dental Medicine, University of Sharjah, Sharjah, UAE.
Eur J Dent. 2018 Oct-Dec;12(4):532-539. doi: 10.4103/ejd.ejd_124_18.
We aim to assess the antibacterial effectiveness of probiotic cleaning in a dental clinic at the University Dental Hospital Sharjah (UDHS), UAE.
The current cleaning protocol of UDHS was evaluated by the surface swabbing of three dental clinics routinely cleaned using regular chemical disinfectants. Then, a new probiotic cleaning solution containing was applied for 3 weeks in a selected clinic. Bacteria were grown onto selective culture media for colony counting from surfaces cleaned with probiotic solution compared to those obtained from the same surfaces cleaned with the regular chemical solutions. Isolates identity was confirmed by biochemical tests or polymerase chain reaction.
There was a significant reduction of the bacterial counts of various bacterial species (, , and Gram-negative rods) from almost all the surfaces in the dental clinic after the application of the probiotic solution compared to the same surfaces cleaned with the regular chemical solutions. However, the antibiotic resistance rates were not significantly reduced within the short period of 3 weeks of using the new probiotic cleaning product.
This study demonstrated that the use of probiotic cleaning is effective in reducing microbial growth in dental settings. This approach may be tested further to examine the long-term effect and to evaluate the opportunity of applying this novel biotechnology as part of the infection control routine in dental settings instead of the chemical disinfectants which are known to cause serious health problems. This is the first study testing the application of probiotic-based solution in dental settings.
我们旨在评估益生菌清洁在阿联酋沙迦大学牙科医院（UDHS）牙科诊所的抗菌效果。
通过对三个常规使用常规化学消毒剂清洁的牙科诊所进行表面擦拭，对UDHS当前的清洁方案进行评估。然后，在一个选定的诊所中应用一种含有[具体成分未给出]的新型益生菌清洁溶液，为期3周。将从用益生菌溶液清洁的表面培养的细菌接种到选择性培养基上进行菌落计数，并与从用常规化学溶液清洁的相同表面获得的细菌进行比较。通过生化试验或聚合酶链反应确认分离株的身份。
与用常规化学溶液清洁的相同表面相比，在牙科诊所几乎所有表面应用益生菌溶液后，各种细菌种类（[具体细菌种类未给出]、[具体细菌种类未给出]和革兰氏阴性杆菌）的细菌计数显著降低。然而，在使用新型益生菌清洁产品的3周短时间内，抗生素耐药率并未显著降低。
本研究表明，使用益生菌清洁可有效减少牙科环境中的微生物生长。这种方法可能需要进一步测试，以检查其长期效果，并评估将这种新型生物技术作为牙科环境感染控制常规措施的一部分来应用的机会，而不是使用已知会导致严重健康问题的化学消毒剂。这是第一项测试基于益生菌的溶液在牙科环境中应用的研究。
