Wang Maolin, Ma Chenyao, Chen Yong, Li Xiang, Chen Jianwei
College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xian Lin Road, Nanjing, 210023, P. R. China.
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing, 210023, P. R. China.
Chem Biodivers. 2019 Feb;16(2):e1800499. doi: 10.1002/cbdv.201800499. Epub 2019 Jan 23.
A new neo-clerodane diterpenoid, barbatin H (1), together with fifteen known analogues (2-16) were isolated from Scutellaria barbata D.Don. Their structures were determined on the basis of NMR and HR-MS spectral analysis and comparison with the reported data. All of those compounds were comparatively predicted for their cytotoxic activities against four human tumor cell lines, i. e. LoVo (colon cancer), MCF-7 (breast cancer), SMMC-7721 (hepatoma cancer), and HCT-116 (colon cancer) cells by MTT method in vitro. The results turned out that the series of neo-clerodane diterpenoids exhibited varying degrees of cytotoxic activities against the growth of the tested tumor cell lines, and most of them exhibited selective cytotoxicity against LoVo cell lines. Scutebata A (14) showed significant cytotoxic activities against four tested tumor cells with IC values of 4.57, 7.68, 5.31, and 6.23 μm, respectively, which indicated that it might be a potential chemotherapeutic agent.
从半枝莲（Scutellaria barbata D.Don）中分离得到一种新的新克罗烷二萜类化合物，barbatin H（1），以及十五种已知类似物（2 - 16）。通过核磁共振（NMR）和高分辨质谱（HR-MS）光谱分析，并与已报道的数据进行比较，确定了它们的结构。采用MTT法在体外对所有这些化合物针对四种人类肿瘤细胞系，即LoVo（结肠癌）、MCF - 7（乳腺癌）、SMMC - 7721（肝癌）和HCT - 116（结肠癌）细胞的细胞毒性活性进行了比较预测。结果表明，该系列新克罗烷二萜类化合物对受试肿瘤细胞系的生长表现出不同程度的细胞毒性活性，且大多数对LoVo细胞系表现出选择性细胞毒性。半枝莲A（14）对四种受试肿瘤细胞均表现出显著的细胞毒性活性，其IC值分别为4.57、7.68、5.31和6.23 μmol/L，这表明它可能是一种潜在的化疗药物。
