Warriar Purnima, Barve Kalyani, Prabhakar Bala
SPP- School of Pharmacy & Technology Management, SVKM's NMIMS, V.L. Mehta Road, Vile Parle (W), Mumbai 400056, Maharashtra, India.
Recent Pat Inflamm Allergy Drug Discov. 2019;13(1):49-56. doi: 10.2174/1872213X12666181120091528.
Garcinia indica also known as kokum is used in traditional system of medicine for relieving inflammation and rheumatic pain. Garcinol, a benzophenone obtained from its fruit rind is reported to have anti-inflammatory effect via modulating arachidonic acid metabolism, suppressing iNOS expression, NF-κB activation and COX-2 expression. It has also been studied for antioxidant and anticancer activity. Apart from these, few patents claim that garcinol also has anti-obesity and hepatoprotective effect and has a potential to be used for the treatment of renal disorders, endometriosis and cardiac dysfunction.
Garcinol Enriched Fraction (GEF) from the fruit rind of Garcinia indica should be effective in the treatment of arthritis, one of the chronic inflammatory disorder owing to its anti-inflammatory property as indicated by earlier experiments.
GEF was prepared from the fruit rind of Garcinia indica and quantified using LC-MS/MS. It was found to contain 89.4% w/w of garcinol. GEF was evaluated at the dose of 10mg/kg for its efficacy against Complete Freund's Adjuvant (CFA) induced arthritis in Wistar albino rats. Paw volumes of both sides were measured by Plethysmometer and body weight was recorded on 0, 1, 5, 12 and 21st day. The hyperalgesic response was also measured by motility test and stair climbing test.
GEF showed a significant reduction in paw swelling (p < 0.0001) and arthritis index (p < 0.0001) exhibiting anti-inflammatory potential. It also improves the motility and stair climbing ability of experimental animals (p < 0.05), thus reducing hyperalgesia.
Garcinol enriched fraction shows anti-arthritic activity in experimental animals.
藤黄果（也称为印度山竹）在传统医学体系中用于缓解炎症和风湿性疼痛。藤黄酚是从其果皮中提取的一种二苯甲酮，据报道它通过调节花生四烯酸代谢、抑制诱导型一氧化氮合酶（iNOS）表达、核因子κB（NF-κB）激活和环氧化酶-2（COX-2）表达而具有抗炎作用。它还被研究用于抗氧化和抗癌活性。除此之外，一些专利声称藤黄酚还具有抗肥胖和肝脏保护作用，并且有潜力用于治疗肾脏疾病、子宫内膜异位症和心脏功能障碍。
鉴于早期实验表明藤黄果的果皮富含藤黄酚的组分（GEF）具有抗炎特性，其应能有效治疗关节炎这一慢性炎症性疾病。
从藤黄果的果皮中制备GEF，并使用液相色谱-串联质谱法（LC-MS/MS）进行定量。发现其含有89.4%（w/w）的藤黄酚。以10mg/kg的剂量评估GEF对Wistar白化大鼠中完全弗氏佐剂（CFA）诱导的关节炎的疗效。在第0、1、5、12和21天通过体积描记器测量双侧爪体积，并记录体重。还通过运动试验和爬楼梯试验测量痛觉过敏反应。
GEF显示爪肿胀（p < 0.0001）和关节炎指数（p < 0.0001）显著降低，表现出抗炎潜力。它还改善了实验动物的运动能力和爬楼梯能力（p < 0.05），从而减轻痛觉过敏。
富含藤黄酚的组分在实验动物中显示出抗关节炎活性。
