Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, Ontario, Canada.
Environ Sci Pollut Res Int. 2019 Jan;26(3):2873-2881. doi: 10.1007/s11356-018-3843-8. Epub 2018 Nov 29.
Silver nanoparticles (AgNPs) were synthesized by incubating the mixture of AgNO solution and whole-cell aqueous extracts (WCAEs) of Neochloris oleoabundans under light conditions. By conducting single-factor and multi-factor optimization, the effects of parameters including AgNO concentration, pH, and extraction time were quantitatively evaluated. The optimal conditions in terms of AgNP yield were found to be 0.8 mM AgNO, pH 5, and 9-h extraction. The AgNPs thus synthesized were quasi-spherical with a mean particle diameter of 16.63 nm and exhibited decent uniformity as well as antibacterial activities, which may facilitate AgNP biosynthesis's application in the near future.
银纳米粒子（AgNPs）是通过在光照条件下孵育硝酸银（AgNO）溶液和油绿叶单胞菌（Neochloris oleoabundans）全细胞水提物（WCAEs）的混合物而合成的。通过进行单因素和多因素优化，定量评估了包括 AgNO 浓度、pH 和提取时间等参数的影响。在 AgNP 产率方面，最佳条件为 0.8 mM AgNO、pH5 和 9 小时提取。所合成的 AgNPs 呈准球形，平均粒径为 16.63nm，具有良好的均一性和抗菌活性，这可能有助于 AgNP 生物合成在不久的将来得到应用。
