Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia.
Institute of Pharmacy, University of Tartu, 50411, Tartu, Estonia.
J Mater Sci Mater Med. 2018 Nov 30;29(12):182. doi: 10.1007/s10856-018-6192-8.
3D fibrous scaffolds have received much recent attention in regenerative medicine. Use of fibrous scaffolds has shown promising results in tissue engineering and wound healing. Here we report the development and properties of a novel fibrous scaffold that is useful for promoting wound healing. A scaffold made of salmon fibrinogen and chitosan is produced by electrospinning, resulting in a biocompatible material mimicking the structure of the native extracellular matrix (ECM) with suitable biochemical and mechanical properties. The scaffold is produced without the need for enzymes, in particular thrombin, but is fully compatible with their addition if needed. Human dermal fibroblasts cultured on this scaffold showed progressive proliferation for 14 days. Split-thickness experimental skin wounds treated and untreated were compared in a 10-day follow-up period. Wound healing was more effective using the fibrinogen-chitosan scaffold than in untreated wounds. This scaffold could be applicable in various medical purposes including surgery, tissue regeneration, burns, traumatic injuries, and 3D cell culture platforms.
3D 纤维支架在再生医学中受到了广泛关注。纤维支架在组织工程和伤口愈合方面显示出了很有前景的结果。在这里，我们报告了一种新型纤维支架的开发和特性，该支架可用于促进伤口愈合。一种由鲑鱼纤维蛋白原和壳聚糖制成的支架是通过静电纺丝制成的，得到的生物相容性材料模拟了天然细胞外基质（ECM）的结构，具有合适的生化和机械性能。该支架的生产不需要酶，特别是凝血酶，但如果需要，完全可以兼容其添加。在该支架上培养的人真皮成纤维细胞在 14 天内表现出渐进性增殖。在 10 天的随访期间，比较了用和不用纤维蛋白原-壳聚糖支架处理的分层实验性皮肤伤口。与未处理的伤口相比，使用纤维蛋白原-壳聚糖支架进行伤口愈合更有效。这种支架可适用于各种医疗用途，包括手术、组织再生、烧伤、创伤和 3D 细胞培养平台。
