Chemistry Department, Federal University of São Carlos, 13565-905 São Carlos, SP, Brazil; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Chemistry Department, Federal University of São Carlos, 13565-905 São Carlos, SP, Brazil.
Talanta. 2019 Apr 1;195:62-68. doi: 10.1016/j.talanta.2018.11.022. Epub 2018 Nov 8.
A new disposable microfluidic electrochemical paper-based device (ePAD) consisting of two spot sensors in the same working electrode for the simultaneous determination of uric acid and creatinine was developed. The spot 1 surface was modified with graphene quantum dots for direct uric acid oxidation and spot 2 surface modified with graphene quantum dots, creatininase and a ruthenium electrochemical mediator for creatinine oxidation. The ePAD was employed to construct an electrochemical sensor (based on square wave voltammetry analysis) for the simultaneous determination of uric acid and creatinine in the 0.010-3.0 µmol L range. The device showed excellent analytical performance with a very low simultaneous detection limit of 8.4 nmol L to uric acid and 3.7 nmol L to creatinine and high selectivity. The ePAD was applied to the rapid and successful determination of those clinical biomarkers in human urine samples.
一种新的一次性微流控电化学纸基装置（ePAD），由同一工作电极上的两个斑点传感器组成，用于同时测定尿酸和肌酐。斑点 1 表面用石墨烯量子点修饰，用于直接氧化尿酸，斑点 2 表面用石墨烯量子点、肌酐酶和钌电化学介体修饰，用于肌酐氧化。该 ePAD 用于构建电化学传感器（基于方波伏安法分析），用于在 0.010-3.0µmol L 范围内同时测定尿酸和肌酐。该装置具有非常低的同时检测限（尿酸为 8.4 nmol L，肌酐为 3.7 nmol L）和高选择性，表现出优异的分析性能。该 ePAD 成功地应用于快速测定人尿液样本中的这些临床生物标志物。
