Bahramian Aghil, Khoshnood Saeed, Shariati Aref, Doustdar Farahnoosh, Chirani Alireza Salimi, Heidary Mohsen
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infect Drug Resist. 2019 Jan 11;12:221-227. doi: 10.2147/IDR.S188527. eCollection 2019.
is the most common opportunistic pathogen associated with a broad range of infections, including cystic fibrosis, ocular, otitis media, and burn infections. The aim of this study was to show the frequency of the gene, and its association with plasmid pKLC102 and PAPI-1 pathogenicity island among strains.
The samples were collected from patients with cystic fibrosis, ocular, otitis media, and burn infections between January 2016 and November 2017. DNA was extracted using the DNA extraction kit and was used for PCR assay. PCR with 4 primer-pairs including 976 F/PAPI-1R, 4542 F/intF, SojR/4541 F, and intF/sojR was performed to identify PAPI-1. pKLC102 was detected using three other primer-pairs including cp10F/cp10R, cp44F/cp44R, and cp97F/cp97R.
A total of 112 . isolates were collected from patients with cystic fibrosis (36), burn (20), otitis media (26), and ocular (30) infections. The results of PCR showed that gene was identified in 96 (85%) strains. PAPI-1-attB integration was detected among 38 (33.9%) isolates and the circular form of PAPI-1 detected among 17 (14%) isolates. In addition, 79 (70.5%) strains were found to be positive for pKLC102.
We found that the majority of the isolates may be susceptible to transfer this significant island and the related element pKLC102 into recipient isolates lacking the island owing to high association of the PilS2 pilus with the islands in the studied strains. It is anticipated that strains isolated from burn and eye with the highest rate of , PAPI-1, and pKLC102 association have a high level of antibiotic resistance.
是与多种感染相关的最常见机会性病原菌，包括囊性纤维化、眼部、中耳炎和烧伤感染。本研究的目的是显示该基因的频率，及其与菌株中质粒pKLC102和PAPI-1致病岛的关联。
2016年1月至2017年11月期间，从患有囊性纤维化、眼部、中耳炎和烧伤感染的患者中采集样本。使用DNA提取试剂盒提取DNA，并用于PCR检测。使用包括976 F/PAPI-1R、4542 F/intF、SojR/4541 F和intF/sojR在内的4对引物进行PCR，以鉴定PAPI-1。使用另外3对引物cp10F/cp10R、cp44F/cp44R和cp97F/cp97R检测pKLC102。
共从囊性纤维化（36例）、烧伤（20例）、中耳炎（26例）和眼部（30例）感染患者中收集到112株分离株。PCR结果显示，96株（85%）菌株中鉴定出该基因。在38株（33.9%）分离株中检测到PAPI-1-attB整合，在17株（14%）分离株中检测到PAPI-1的环状形式。此外，79株（70.5%）菌株pKLC102检测呈阳性。
我们发现，在所研究的菌株中，由于PilS2菌毛与这些岛高度相关，大多数分离株可能易于将这个重要的岛和相关元件pKLC102转移到缺乏该岛的受体分离株中。预计从烧伤和眼部分离出的、与该基因、PAPI-1和pKLC102关联率最高的菌株具有高水平的抗生素耐药性。
