Florida Medical Entomology Laboratory, University of Florida, 200 9th Street South East, Vero Beach, FL 32962, USA.
Int J Mol Sci. 2019 Jan 31;20(3):615. doi: 10.3390/ijms20030615.
(L.) is the primary vector of chikungunya, dengue, yellow fever, and Zika viruses. The leucine-rich repeats (LRR)-containing domain is evolutionarily conserved in many proteins associated with innate immunity in invertebrates and vertebrates, as well as plants. We focused on the and gene expressions in response to Zika virus (ZIKV) and chikungunya virus (CHIKV) infection using a time course study, as well as the developmental expressions in the eggs, larvae, pupae, and adults. RNA-seq analysis data provided 60 leucine-rich repeat related transcriptions in in response to Zika virus (Accession number: GSE118858, accessed on: August 22, 2018, GEO DataSets). RNA-seq analysis data showed that (AAEL012086-RA) and (AAEL009520-RA) were significantly upregulated 2.5 and 3-fold during infection by ZIKV 7-days post infection (dpi) of an Key West strain compared to an Orlando strain. The qPCR data showed that LRR-containing proteins related genes, and , and five paralogues were expressed 100-fold lower than other nuclear genes, such as defensin, during all developmental stages examined. Together, these data provide insights into the transcription profiles of LRR proteins of during its development and in response to infection with emergent arboviruses.
（L.）是基孔肯雅热、登革热、黄热病和寨卡病毒的主要载体。富含亮氨酸重复序列（LRR）的结构域在无脊椎动物和脊椎动物以及植物中与先天免疫相关的许多蛋白质中是进化保守的。我们专注于 和 基因在寨卡病毒（ZIKV）和基孔肯雅热病毒（CHIKV）感染时的表达，采用时间过程研究，以及在卵、幼虫、蛹和成虫中的发育表达。RNA-seq 分析数据提供了 60 个与寨卡病毒（注册号：GSE118858，访问日期：2018 年 8 月 22 日，GEO 数据集）反应的 LRR 相关转录物在 中。RNA-seq 分析数据显示，与奥兰多株相比，ZIKV 感染 7 天后（感染后 7 天）， （AAEL012086-RA）和 （AAEL009520-RA）的表达分别上调了 2.5 倍和 3 倍。qPCR 数据显示，在所有检测的发育阶段，富含 LRR 的蛋白相关基因 、 、 和五个旁系同源基因的表达水平比防御素等其他核基因低 100 倍。总之，这些数据提供了在其发育过程中和对新兴虫媒病毒感染时 LRR 蛋白的转录谱的深入了解。
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