果蝇视觉系统在突触形成过程中细胞类型特异性的无刺激诱导的神经元活动。
Cell-type-Specific Patterned Stimulus-Independent Neuronal Activity in the Drosophila Visual System during Synapse Formation.
机构信息
Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Neuron. 2019 Mar 6;101(5):894-904.e5. doi: 10.1016/j.neuron.2019.01.008. Epub 2019 Jan 30.
Stereotyped synaptic connections define the neural circuits of the brain. In vertebrates, stimulus-independent activity contributes to neural circuit formation. It is unknown whether this type of activity is a general feature of nervous system development. Here, we report patterned, stimulus-independent neural activity in the Drosophila visual system during synaptogenesis. Using in vivo calcium, voltage, and glutamate imaging, we found that all neurons participate in this spontaneous activity, which is characterized by brain-wide periodic active and silent phases. Glia are active in a complementary pattern. Each of the 15 of over 100 specific neuron types in the fly visual system examined exhibited a unique activity signature. The activity of neurons that are synaptic partners in the adult was highly correlated during development. We propose that this cell-type-specific activity coordinates the development of the functional circuitry of the adult brain.
刻板的突触连接定义了大脑的神经回路。在脊椎动物中，与刺激无关的活动有助于神经回路的形成。目前尚不清楚这种类型的活动是否是神经系统发育的普遍特征。在这里，我们报告了在果蝇视觉系统的突触发生过程中存在有模式的、与刺激无关的神经活动。通过在体钙、电压和谷氨酸成像，我们发现所有神经元都参与了这种自发活动，其特征是全脑周期性的活跃和沉默阶段。神经胶质细胞以互补的模式活跃。在果蝇视觉系统中检查的 100 多种特定神经元类型中的每一种都表现出独特的活动特征。在发育过程中，成年后成为突触伙伴的神经元的活动具有高度相关性。我们提出，这种细胞类型特异性的活动协调了成年大脑功能回路的发育。
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