Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Department of Orthodontics, Institute of Craniofacial Deformity, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Sci Rep. 2019 Feb 5;9(1):1432. doi: 10.1038/s41598-018-38255-2.
We evaluated the efficacy of light-curable fluoride varnish (LCFV) that contains 2-methacryloyloxyethyl phosphorylcholine (MPC) in terms of anti-biofouling properties and prevention of tooth enamel demineralization. MPC was mixed with and incorporated into LCFV at 0 (control), 1.5, 3.0, 5.0, 10.0, 20.0, and 40.0 weight percentage (wt%). Addition of high wt% of MPC resulted in increased film thickness and decreased the degree of conversion, indicating loss of the advantageous properties of LCFV. Addition of 1.5, 3, or 5 wt% MPC significantly reduced the amount of bovine serum albumin adsorbed from a solution and proteins adsorbed from brain heart infusion medium compared to the control (P < 0.001). A similar pattern was observed for bacterial adhesion: significantly less Streptococcus mutans cells adhered on the surface of LCFV with 1.5, 3, or 5 wt% MPC (P < 0.001) than on the control, and similar results were obtained for Actinomyces naeslundii and Streptococcus sanguinis adherence to LCFV with 3 wt% MPC. Finally, bacterial adhesion, surface microhardness loss, and the depth of demineralization were substantially lower on bovine tooth enamel surface coated with LCFV containing 3 wt% of MPC than in the control treatment (0 wt% MPC). Therefore, this novel LCFV containing a low concentration of MPC (e.g., 3 wt%) would be effective in anti-biofouling while maintaining the important advantageous features of light-curable fluoride in preventing demineralization.
我们评估了含有 2-（甲基丙烯酰氧基）乙基磷酰胆碱（MPC）的光固化氟化物漆（LCFV）在抗生物污损特性和预防牙釉质脱矿方面的功效。MPC 与 LCFV 以 0（对照）、1.5、3.0、5.0、10.0、20.0 和 40.0 重量百分比（wt%）混合并掺入。添加高 wt%的 MPC 会导致膜厚度增加和转化率降低，表明 LCFV 的有利特性丧失。添加 1.5、3 或 5wt%的 MPC 可显著减少从溶液中吸附的牛血清白蛋白的量和从脑心浸液培养基中吸附的蛋白质量，与对照相比（P<0.001）。对于细菌粘附也观察到类似的模式：与对照相比，在 LCFV 表面上粘附的变形链球菌细胞数量显著减少，添加 1.5、3 或 5wt%的 MPC（P<0.001），对于粘附到含有 3wt%MPC 的 LCFV 的奈瑟氏放线菌和血链球菌也得到了类似的结果。最后，在涂有含 3wt%MPC 的 LCFV 的牛牙釉质表面上，细菌粘附、表面显微硬度损失和脱矿深度均明显低于对照处理（0wt%MPC）。因此，这种新型的 LCFV 含有低浓度的 MPC（例如 3wt%），在保持光固化氟化物在预防脱矿方面的重要有利特性的同时，在抗生物污损方面将是有效的。
