CBQF-Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital, 4200-072, Porto, Portugal.
3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradable and Biomimetics, Headquarters of the European Institute of Excellence on tissue Engineering and Regenerative Medicine, AvePark, 4805-17, Barco, Guimarães, Portugal.
J Mater Sci Mater Med. 2019 Feb 12;30(2):27. doi: 10.1007/s10856-019-6225-y.
Guided tissue regeneration (GTR) is a surgical procedure applied in the reconstruction of periodontal defects, where an occlusive membrane is used to prevent the fast-growing connective tissue from migrating into the defect. In this work, silk fibroin (SF) membranes were developed for periodontal guided tissue regeneration. Solutions of SF with glycerol (GLY) or polyvinyl alcohol (PVA) where prepared at several weight ratios up to 30%, followed by solvent casting and thermal annealing at 85 °C for periods of 6 and 12 h to produce high flexible and stable membranes. These were characterized in terms of their morphology, physical integrity, chemical structure, mechanical and thermal properties, swelling capability and in vitro degradation behavior. The developed blended membranes exhibited high ductility, which is particular relevant considering the need for physical handling and adaptability to the defect. Moreover, the membranes were cultured with human periodontal ligament fibroblast cells (hPDLs) up to 7 days. Also, the higher hydrophilicity and consequent in vitro proteolytic degradability of these blends was superior to pure silk fibroin membranes. In particular SF/GLY blends demonstrated to support high cell adhesion and viability with an adequate hPDLs' morphology, make them excellent candidates for applications in periodontal regeneration.
引导组织再生（GTR）是一种应用于牙周缺损重建的外科手术，其中使用封闭膜来防止快速生长的结缔组织迁移到缺损中。在这项工作中，丝素（SF）膜被开发用于牙周引导组织再生。将丝素与甘油（GLY）或聚乙烯醇（PVA）在几个重量比下制备成溶液，直至 30%，然后进行溶剂浇铸和 85°C 下热退火 6 和 12 小时，以生产出高柔韧性和稳定的膜。这些膜在形态、物理完整性、化学结构、机械和热性能、溶胀能力和体外降解行为方面进行了表征。所开发的共混膜表现出高延展性，这在考虑到物理处理的需要和对缺陷的适应性方面尤为重要。此外，将这些膜与人牙周韧带成纤维细胞（hPDLs）共培养了 7 天。此外，这些共混物具有更高的亲水性和随后的体外蛋白水解降解性，优于纯丝素膜。特别是 SF/GLY 共混物被证明能够支持高细胞黏附和活力，同时保持 hPDLs 的适当形态，使它们成为牙周再生应用的优秀候选材料。
