Department of Chemistry , Purdue University , West Lafayette , Indiana 47907 , United States.
ACS Appl Mater Interfaces. 2019 Apr 17;11(15):13853-13858. doi: 10.1021/acsami.8b22691. Epub 2019 Feb 22.
We report a nanopatterning strategy in which self-assembled DNA nanostructures serve as structural templates. In previous work, ordering of NPs primarily relied on specific recognition, e.g., DNA-DNA hybridization. Only a few cases have been reported on nonspecific adsorption. Unfortunately, these studies were limited by the integrity and homogeneity of templates and the variety of patterned nanoparticles (NPs). Herein, we have developed a general method to pattern various NPs. The NPs adsorb onto substrate via NP-substrate direct interactions and the substrates are patterned into large arrays (>4 × 4 μm) of tiny, accessible cavities by self-assembled DNA arrays. As a demonstration, DNA templates include tetragonal and hexagonal arrays and the NPs include individual DNA nanomotifs, gold nanoparticles (AuNPs), and proteins. All nanostructures have been confirmed by atomic force microscopy and corresponding fast Fourier transform (FFT) analysis.
我们报告了一种纳米图案化策略，其中自组装 DNA 纳米结构作为结构模板。在以前的工作中， NPs 的有序排列主要依赖于特定的识别，例如 DNA-DNA 杂交。只有少数几个非特异性吸附的例子被报道。不幸的是，这些研究受到模板的完整性和均一性以及图案化纳米颗粒 (NPs) 的多样性的限制。在这里，我们开发了一种通用的方法来对各种 NPs 进行图案化。 NPs 通过 NP-基底直接相互作用吸附到基底上，并且基底通过自组装 DNA 阵列被图案化成具有微小、可及的腔的大阵列 (>4×4 μm)。作为一个例子，DNA 模板包括四方和六角形阵列，而 NPs 包括单个 DNA 纳米马达、金纳米粒子 (AuNPs) 和蛋白质。所有的纳米结构都已经通过原子力显微镜和相应的快速傅里叶变换 (FFT) 分析得到了证实。
