Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, Urbana, IL, USA.
Division of Nutritional Sciences, University of Illinois, Urbana-Champaign, Urbana, IL, USA.
Nutr Neurosci. 2020 Dec;23(12):983-992. doi: 10.1080/1028415X.2019.1582578. Epub 2019 Feb 22.
Habitual diet impacts mood and the human gastrointestinal (GI) microbiota. Yet, studies infrequently control for diet when evaluating associations between mood and GI microbiota. Accordingly, we aimed to investigate relationships among diet, GI microbiota, and mood in adults without mood disorders by conducting a cross-sectional examination of dietary intake, subjective emotional state, and fecal microbial taxa abundances. Adults ( = 133; 25-45 years of age) without physician-diagnosed mood disorders were studied. Fecal DNA was extracted, and the V4 region of the 16S rRNA gene was sequenced. Sequences were analyzed using QIIME2. Subjective mood state was assessed using the 42-item Depression, Anxiety, and Stress Scale (DASS-42). Habitual dietary intake was measured with the National Cancer Institute's Diet History Questionnaire II, and diet quality was evaluated with the 2010 Healthy Eating Index (HEI). Relationships were observed between 28 bacterial taxa and DASS-42 scores. Sex-dependent associations were observed among 21 bacterial taxa and DASS-42 scores, including an inverse relationship between Anxiety scale scores and in females and an inverse relationship between Depression scale scores and in males. HEI total fruit and dairy components were inversely associated with Depression and Stress scales, respectively. These results suggest GI microbes are related to mood in adults without diagnosed mood disorders and that these relationships differ by sex and are influenced by dietary fiber intake. Incorporating dietary intake data in gut-microbiota-brain studies may help clarify the roles of specific microbes and dietary components in mental health symptoms.
习惯性饮食会影响情绪和人体胃肠道（GI）微生物群。然而，在评估情绪和 GI 微生物群之间的关联时，研究很少控制饮食。因此，我们通过对饮食摄入、主观情绪状态和粪便微生物分类群丰度进行横断面检查，旨在研究无情绪障碍的成年人中饮食、GI 微生物群和情绪之间的关系。研究对象为（ = 133；年龄 25-45 岁）无医生诊断情绪障碍的成年人。提取粪便 DNA，并对 16S rRNA 基因的 V4 区进行测序。使用 QIIME2 对序列进行分析。使用 42 项抑郁、焦虑和压力量表（DASS-42）评估主观情绪状态。习惯性饮食摄入通过国家癌症研究所饮食史问卷 II 进行测量，饮食质量通过 2010 年健康饮食指数（HEI）进行评估。28 种细菌分类群与 DASS-42 评分之间存在相关性。在 21 种细菌分类群和 DASS-42 评分之间观察到性别依赖性关联，包括女性焦虑量表评分与 呈负相关，男性抑郁量表评分与 呈负相关。HEI 总水果和乳制品成分与抑郁和压力量表呈负相关。这些结果表明，GI 微生物与无诊断情绪障碍的成年人的情绪有关，这些关系因性别而异，并且受膳食纤维摄入量的影响。在肠道微生物群-大脑研究中纳入饮食摄入数据可能有助于阐明特定微生物和饮食成分在心理健康症状中的作用。
