Shimizu Hideyuki, Kaneko Hiroki, Suzumura Ayana, Takayama Kei, Namba Rina, Funahashi Yasuhito, Kataoka Keiko, Iwase Takeshi, Hwang Shiang-Jyi, Ito Seina, Yamada Kazuhisa, Ueno Shinji, Ito Yasuki, Terasaki Hiroko
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Ophthalmology, National Defense Medical College, Nagoya, Japan.
Transl Vis Sci Technol. 2019 Feb 28;8(1):28. doi: 10.1167/tvst.8.1.28. eCollection 2019 Jan.
Subsilicone oil fluid (SOF) in eyes with silicone oil (SO) endotamponade possibly has a role in complications (e.g., vision loss); thus, we aimed to examine inflammatory cytokine and electrolyte levels and retinal glial cell viability in SOF.
We measured major inflammatory cytokine levels and electrolytes in SOF and compared them with those in vitreous fluid (VF) and anterior chamber fluid (ACF). We analyzed the correlation between inflammatory cytokines and retinal thickness in SO-filled eyes. Further, we measured the MIO-M1 cell viability in medium with SOF and compared it with that containing VF.
We collected and examined 57 SOF, 22 ACF, and 21 VF samples from eyes with PVR, PDR, RD, and MH. Interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 levels in SOF were significantly higher than those in ACF. There was no significant difference for all cytokines between SOF and VF. Retinal thickness changes during SO endotamponade were not correlated with the presence of any inflammatory cytokines. Levels of ferrous iron, but not of potassium, showed a significant decrease in SOF compared with VF. The WST-1 assay showed that SOF-added medium induced higher MIO-M1 cell viability than VF-added medium.
We found no significant correlation between the change in the retinal thickness and cytokine levels, but SOF contains higher concentrations of cytokines and lower concentrations of ferrous iron and can be biologically distinguished from ACF and VF.
Novel knowledge of inflammatory cytokine levels and electrolytes in SOF provides better understanding of pathology of SO-filled eyes.
在接受硅油（SO）内填塞的眼中，硅油下积液（SOF）可能与并发症（如视力丧失）有关；因此，我们旨在检测SOF中的炎性细胞因子和电解质水平以及视网膜神经胶质细胞的活力。
我们测量了SOF中的主要炎性细胞因子水平和电解质，并将其与玻璃体液（VF）和前房液（ACF）中的水平进行比较。我们分析了接受SO填充的眼中炎性细胞因子与视网膜厚度之间的相关性。此外，我们测量了在含有SOF的培养基中MIO-M1细胞的活力，并将其与含有VF的培养基中的细胞活力进行比较。
我们从患有增殖性玻璃体视网膜病变（PVR）、增殖性糖尿病视网膜病变（PDR）、视网膜脱离（RD）和黄斑裂孔（MH）的眼中收集并检测了57份SOF样本、22份ACF样本和21份VF样本。SOF中的白细胞介素（IL）-8和单核细胞趋化蛋白（MCP）-1水平显著高于ACF中的水平。SOF和VF之间所有细胞因子均无显著差异。SO内填塞期间视网膜厚度的变化与任何炎性细胞因子的存在均无相关性。与VF相比，SOF中亚铁离子水平显著降低，但钾离子水平无显著变化。WST-1检测显示，添加SOF的培养基比添加VF的培养基诱导更高的MIO-M1细胞活力。
我们发现视网膜厚度变化与细胞因子水平之间无显著相关性，但SOF含有较高浓度的细胞因子和较低浓度的亚铁离子，并且在生物学上可与ACF和VF区分开来。
关于SOF中炎性细胞因子水平和电解质的新知识有助于更好地理解接受SO填充的眼的病理情况。
