Department of Chemistry, Duke University, Durham, NC, United States of America. Department of Biomedical Engineering, Duke University, Durham, NC, United States of America.
Nanotechnology. 2019 Jul 5;30(27):275101. doi: 10.1088/1361-6528/ab0e9c. Epub 2019 Mar 11.
Despite decades of efforts, non-invasive sensitive detection of small malignant brain tumors still remains challenging. Here we report a dual-modality I-labeled gold nanostar (I-GNS) probe for sensitive brain tumor imaging with positron emission tomography (PET) and subcellular tracking with two-photon photoluminescence (TPL) and electron microscopy (EM). Experiment results showed that the developed nanoprobe has potential to reach sub-millimeter intracranial brain tumor detection using PET scan, which is superior to any currently available non-invasive imaging modality. Microscopic examination using TPL and EM further confirmed that GNS nanoparticles permeated the brain tumor leaky vasculature and accumulated inside brain tumor cells following systemic administration. Selective brain tumor targeting by enhanced permeability and retention effect and ultrasensitive imaging render I-GNS nanoprobe promise for future brain tumor-related preclinical and translational applications.
尽管经过了几十年的努力，对小的恶性脑肿瘤的非侵入性敏感检测仍然具有挑战性。在这里，我们报告了一种双模态 I 标记的金纳米星（I-GNS）探针，用于正电子发射断层扫描（PET）的敏感脑肿瘤成像和双光子光致发光（TPL）和电子显微镜（EM）的亚细胞跟踪。实验结果表明，所开发的纳米探针具有使用 PET 扫描达到亚毫米颅内脑肿瘤检测的潜力，优于任何当前可用的非侵入性成像方式。使用 TPL 和 EM 的显微镜检查进一步证实，GNS 纳米颗粒穿透了脑肿瘤的渗漏血管，并在系统给药后积聚在脑肿瘤细胞内。通过增强的通透性和保留效应以及超灵敏成像实现的选择性脑肿瘤靶向，使 I-GNS 纳米探针有望用于未来与脑肿瘤相关的临床前和转化应用。
